正交设计优化胚胎期抗手机辐射方剂研究  

Study on the optimization of anti-radiation formula during embryonic stage by orthogonal design

作　　者：高辉[1] 裴银辉[2] 孙君侠[3] 李锋[1] 

机构地区：[1]唐山职业技术学院临床医学系,河北唐山063004 [2]河北联合大学 [3]唐山协和医院

出　　处：《中国妇幼保健》2012年第32期5169-5172,共4页Maternal and Child Health Care of China

基　　金：河北省2010年科技支撑计划课题〔10276128〕

摘　　要：目的:通过正交设计优化胚胎期抗手机辐射方剂。方法:以小鼠为受试对象,应用两次正交设计,以外周血白细胞总数、骨髓有核细胞数和骨髓细胞微核率为评价指标,选择胚胎期抗手机辐射最佳药物和剂量组合。结果:通过第1次正交设计,得到最佳抗辐射药物组合为菊花、决明子和玄参;第2次正交设计得到上述药物最佳剂量分别为6、9和9 g。结论:通过两次正交设计,优化胚胎期抗手机配伍为菊花6 g、决明子9 g、玄参9 g。Objective： To optimize the anti -radiation formula during embryonic stage by orthogonal design. Methods： Experimental mouse was severed as survey, and twice orthogonal designs were performed for the selection of the formula. Counts of white blood cells in peripheral and nuclear cells in bone marror were detected to evaluate the effect of anti - radiation, so the percentage of micronuclear cells in bone marrow. Results： Attributed to the first orthogonal design, the optimal anti - radiation formula during embryonic stage was chrysan- themum, cassiac torae and figwort. After the second orthogonal design was performed, the optimal doses of the upper 3 herbs were 6g, 9g and 9g respectively. Conclusion： The optimal anti -radiation formula during embryonic stage obtained by twice orthogonal designs is as the following, 6g of chrysanthemum, 9g of cassiac torae and 9g of figwort.

关 键 词：正交设计 优化 抗辐射 胚胎期 

分 类 号：R715[医药卫生—妇产科学]