青枯病和根肿病生防细菌BS2004的发酵培养基和培养条件优化  

Optimization of Medium and Cultural Conditions for Biocontrol Bacterial Strain BS2004 against Ralstonia solanacearum and Plasmodiophora brassicae

作　　者：周青[1] 王笑 苏婷[1] 路梅[3] 谢关林[1] 

机构地区：[1]浙江大学水稻生物学国家重点实验室,浙江大学生物技术研究所,杭州310029 [2]浙江省宁波市江北区农技推广服务总站 [3]浙江省师范大学

出　　处：《中国生物防治学报》2012年第4期537-544,共8页Chinese Journal of Biological Control

基　　金：农业部公益性行业(农业)科研专项(201003029;201003066);国家自然科学基金(30871655)

摘　　要：解淀粉芽孢杆菌BS2004是一株对不同生化型的番茄青枯病菌Ralstonia solanacearum和十字花科根肿病菌Plasmodiophora brassicae具有抑制作用的优良生防菌,主要依靠产生拮抗物质抑制病菌。为提高其抑菌活性物质的产量与防治效果,本文采用正交试验和单因素试验设计,通过摇瓶培养对其发酵培养基和培养条件进行了优化。优化后的发酵培养基配方为蛋白胨10 g.L 1、牛肉浸膏1 g.L 1、酵母粉1 g.L 1和Nacl 8 g.L 1;优化后的培养条件为pH 7.5、温度35℃、装瓶量35 mL.50 mL 1、接种量3%(v/v)和最佳培养时间24 h。经条件优化后培养的BS2004对青枯病菌的抑菌圈直径达21.75 mm,对番茄青枯病的防治效果达80%,对十字花科根肿病的防治效果达60%,增产1.92倍。Antagonistic Bacillus amyloliquefaciens BS2004 was a promising biocontrol agent against various biotypes of Ralstonia solanacearum and Plasmodiophora brassicae by largely producing antagonistic substances. To improve the yield of its active substances and control effect, the medium and cultural conditions of strain BS2004 were optimized by orthogonal design method and single factor tests in shake-flask. Results showed that the optimal medium were composed of 10 g peptone, beef extract 1 g, yeast extract 1 g, and NaC1 8 g in 1 L medium. The optimal cultural conditions were as follows： initial pH 7.5, temperature 35 ℃, medium volume 35 mL in 50 mL flask, inoculum volume 3% （v/v） and fermentation time 24 h. Under the conditions, the diameter of inhibition zone between the bacteria and R. solanacearum reached 21.75 mm and control effect of the strain against tomato bacterial wilt reached 80%. The control effect of strain BS2004 against crucifer club root disease was up to 60% and the yield increased 1.92 times.

关 键 词：青枯菌 根肿菌 生防菌BS2004 正交设计 发酵培养基 培养条件 优化 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治] TQ920.6[农业科学—植物保护]