表面活性剂和染料探针共振光散射法测定蛋白质  

Determination of protein with surfactant and dye probe by resonance light scattering technique

作　　者：张亚南[1] 冯素玲[1] 

机构地区：[1]河南师范大学化学与环境科学学院,河南新乡453007

出　　处：《化学研究与应用》2012年第11期1624-1628,共5页Chemical Research and Application

摘　　要：在pH为2.1的Britton-Robinson缓冲溶液中,蛋白质与十二烷基磺酸钠和吖啶红作用形成聚集体,导致体系产生较大的共振散射光(RLS)强度,并且RLS的增强程度与蛋白质的浓度呈线性关系,据此建立了测定蛋白质含量的方法。人血清白蛋白(HSA)和牛血清白蛋白(BSA)分别在0.03～3.0μg/mL和0.2～7.0μg/mL的浓度范围内与增强的共振光散射强度有良好的线性关系,相应的检测限分别为0.0119μg/mL和0.026μg/mL。对体系的反应机理进行了讨论。将方法用于人血清中蛋白质含量的测定,并将分析结果与考马斯亮蓝法进行了对照。t-检验证明,两种方法无显著性差异。It is found that sodium dodecyl sulfonate and acridine red combine with protein to form an aggregate at pH 2. 10. It results in a significant enhancement of the resonance light scattering（RLS） ,and the enhancement degree of RLS is linear with the concen- tration of protein. Based on it, a method for determination of protein has been developed, different proteins and the enhancement of RLS have good linear relationship in the range of 0. 030 - 3.0μg/mL for human serum albumin,0. 20 -7.0μg/mL for bovine serum albumin, the corresponding detection limits are O. 0119μg/mL 和0. 026pxg/mL respectively. The reaction mechanism of system is discussed. The method is used for analyzing protein in human serum, and the detection results are compared with those of Coomassie brilliant blue method, there are no significant differences between two methods according to the t-test.

关 键 词：共振光散射 吖啶红 十二烷基磺酸钠 蛋白质 

分 类 号：O657.3[理学—分析化学]