去卵巢和接触铅对大鼠骨和血液影响的比较实验  

Comparative study of impacts of ovariotomy and lead exposure on rat bone and blood

作　　者：方晓燕[1] 李海[1] 王金花[1] 陈建海[1] 黎飚[1] 张树球[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《工业卫生与职业病》2012年第6期342-345,共4页Industrial Health and Occupational Diseases

基　　金：广西自然科学基金(2010GXNSFA013268);右江民族医学院科研课题基金(yk2009002)

摘　　要：目的了解雌激素缺乏和铅中毒对大鼠骨组织和血液影响,为绝经后妇女骨质疏松症和铅中毒所引起的骨质疏松症的防治提供实验依据。方法选取5月龄健康雌性大鼠60只,随机分为对照组、去卵巢模型(OVX)组和铅中毒模型(LPD)组。12周后,取大鼠眼球静脉血,处死取其股骨进行骨组织切片形态观察,并对骨组织和血液中的钙、磷、铅和碱性磷酸酶进行测定,数据进行统计学分析。结果对照组骨组织和血红蛋白正常;OVX组和LPD组中骨钙、骨磷、血钙及血磷均降低(P<0.01),血中碱性磷酸酶活力升高(P<0.01),骨组织形态呈现骨质疏松的病理改变。LPD组的骨铅和血铅明显高于对照组(P<0.01)。结论去卵巢大鼠雌激素缺乏引起骨质疏松症,铅中毒也可引起骨质疏松症,并使血红蛋白含量降低。Objective To understand the impacts of estrogen deficiency and lead exposure on osteoporosis and finally to provide preventive criteria for osteoporosis induced by menopause and lead exposure. Methods Sixty 5 months old female rats were equally divided into three groups, i. e. the control group, after ovariotomy group, and lead exposed group. After 12 weeks, eye vein blood and a section of femur were taken. Morphology of bone tissue was investigated; calcium, phosphorus, lead and alkaline phosphatase in blood and bone were measured. Data were statistically analyzed. Results Bone tissue and hemoglobin in the control group were normal. Both calcium and phosphorus in the bone and blood in either after ovariotomy group and lead exposed group were lowered, P〈 0.01, while blood alkaline phosphatase increased, P 〈0. 01. Pathological changes of osteoporosis were found. Lead contents in both bone tissue and blood of lead exposed group were significantly higher than those of the control group（P〈0.01）. Conclusions Estrogen deficiency after ovariotomy may induce osteoporosis; lead exposure may also induce osteoporosis, at the same time causing hemoglobin lowered.

关 键 词：去卵巢大鼠 铅中毒大鼠 骨组织 血液 

分 类 号：R135.1[医药卫生—劳动卫生]