肾上腺髓质和嗜铬细胞植入蛛网膜下腔后的存活能力比较  

Comparison of viabilities of homologous adrenal medullary pieces and isolated chromaffin cells following implantation into subarachnoid space of rat

作　　者：杨茂元[1] 李宏莲[2] 毕好生[1] 田玉科[1] 向桂芳[1] 丁素琴[1] 

机构地区：[1]同济医科大学附属同济医院麻醉学教研室,武汉430030 [2]同济医科大学组织胚胎学教研室

出　　处：《中华麻醉学杂志》2000年第5期280-282,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学研究基金! ( 3990 0 139);卫生部科学研究基金 !( 98 1 12 9)

摘　　要：目的　评估同种异体肾上腺髓质碎片和游离嗜铬细胞在脑脊液这一特殊环境中的存活能力 ,为椎管腔移植细胞镇痛技术的论证提供形态学依据。方法　雄性SD大鼠 40只 ,体重 180 -2 2 0 g ,随机均分为两组 ,分别向蛛网膜下腔植入肾上腺髓质碎片 (A组 )和游离嗜铬细胞 (B组 )。移植物在移植前均经过 3d的体外培养。肾上腺髓质碎片经PBS溶液冲洗后直接植入蛛网膜下腔 ,游离嗜铬细胞则以悬浮液 10 μl(含细胞 5× 10 7)注入。移植术前、移植术后第 5周和第 10周测定大鼠的热痛阈。移植术后第 5周和第 10周 ,每组随机取 6只大鼠 ,麻醉后自脊髓腔取髓质碎片或脑脊液沉淀作电镜切片观察。结果　两组动物于术后第 5周的热痛阈均明显高于基础值 (P <0 .0 5 ) ,两组间无显著性差异 (P >0 .0 5 )。电镜切片显示均可见完整的嗜铬细胞。第 10周 ,A组动物的热痛阈降至基础值水平 (P >0 .0 5 ) ,明显低于B组值 (P <0 .0 5 )。A组切片上未见完整的嗜铬细胞而伴有大量散在的淋巴细胞浸润 ;B组切片上尚可见形态完整的嗜铬细胞 ,有少量的淋巴细胞浸润 ,但数量及密度均低于A组。结论　游离嗜铬细胞比肾上腺髓质碎片 。Objective To compare the survival of isolated chromaffin cells with that of adrenal medullary pieces implanted into the subarachnoid space .Methods Forty male Sprague Dawley rats, 180 220g, were randomly allocated to be implanted into the subarachnoid space with the homologous adrenal medullary pieces washed with PBS(group A,n=20) or the isolated chromaffin cells 5×107 in 10μl suspension (group B,n=20), respectively, after the implants were in vitro cultured in three days . The thermal threshold was determined before ,5 and 10 weeks after the transplantation. Five or ten weeks after the transplantation, 6 rats of either group were randomly selected to obtain the adrenal medullary grafts from the subarachnoid space or the sediments of cerebrospinal fluid ,observed with an electron microscopy.Results Five weeks after transplantation, the thermal threshold in both groups increased markedly,but without significant difference between them ,and there were intact chromaffin cells in the sections of both groups. Ten weeks after the operation, the thermal threshold in group A decreased to basaline, and was significantly lower than in group B; no intact chromaffin cells were found ,with the infiltration of a large number lymphocytes in the sections of group A , and in the sections of group B, the intact chromaffin cells were found with the infiltration of lymphocytes in lower amount and density. Conclusions The isolated chromaffin cells implantation is superior to the adrenal medulla pieces implantation for analgesia.

关 键 词：嗜铬细胞 肾上腺髓质 移植 蛛网膜下腔 存活能力 

分 类 号：R456[医药卫生—治疗学]