中国鄂温克、鄂伦春、达斡尔族永生细胞系的建立与保存  

Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the Ewenki,the Oroqen and the Daur Ethnic Groups in China

作　　者：薛雅丽[1] 王琦[1] 史忠诚[1] 刘岸[1] 张钰[1] 黄小义[1] 黄承滨[1] 陈白滨[1] 杨焕杰[1] 傅松滨[1] 李璞[1] 

机构地区：[1]哈尔滨医科大学医学遗传学研究室,黑龙江哈尔滨150086

出　　处：《遗传》2000年第3期157-158,共2页Hereditas（Beijing)

基　　金：国家自然科学基金资助项目 ;黑龙江省科学技术攻关项目

摘　　要：本文采用EBV(Epstein BarrVirus)上清液转化B淋巴细胞 ,并加入环胞霉素A(CyclosporineA)抑制T淋巴细胞 ,成功地对中国东北地区鄂温克族、鄂伦春族及达斡尔族的部分个体建立了永生细胞系 ,其中鄂温克族49株 ,鄂伦春族40株 ,达斡尔族51株 ,总计140株。永久保存我国特有民族的基因组 。The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhabit the activity of T cells.In the present study ,140 immortal cell lines of the Ewenki,the Oroqen and the Daur ethnic groups in the Northeast China were established .This is an important part of the research of human genome diversity for the exploration of the origin and progression of different ethnic groups ,and also provide enough research materials for further studies.

关 键 词：永生细胞系 EBV 少数民族 鄂温克族 鄂伦春族 

分 类 号：Q987[生物学—遗传学]