图位克隆技术在分离植物基因中的应用  

The Application of Map-based Gene Cloning Method on Isolating Genes of Plants

作　　者：景润春[1] 黄青阳[1] 朱英国[1] 

机构地区：[1]武汉大学发育生物学研究中心武汉大学遗传研究所,武汉430072

出　　处：《遗传》2000年第3期180-185,共6页Hereditas（Beijing)

基　　金：国家自然科学基金资助项目!(39770455)

摘　　要：本文综述图位克隆技术的原理及基本技术环节 ,并与其它基因克隆技术相比较说明图位克隆技术的优缺点。本文还对近年来图位克隆技术在分离不同的植物发育基因上的广泛应用进行了简要概述 ,并对其应用前景和近期的预期进展作出展望。In this review,the principle and basis steps of map based gene cloning on plant were introduced in detail.At the same time,the advantages and disadvantage of the method were evaluated as compared with the other methods.The extensive application of the map based gene cloning method on cloning genes of different plants in the past was also summarized in brief.The prospect and the expected progress in several years were proposed in this paper.

关 键 词：图位克隆 基因定位 物理图谱 比较作图 植物基因 

分 类 号：Q943.2[生物学—植物学]