甜叶菊渣中总黄酮的提取工艺优选  

Optimization of Extraction Process for Total Flavonoids from Residues of Stevia rebaudiana

作　　者：史高峰[1] 王红玉[1] 王国英[1] 宫建辉[1] 王振菊[1] 

机构地区：[1]兰州理工大学石油化工学院,兰州730050

出　　处：《中国实验方剂学杂志》2012年第22期62-64,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选甜叶菊渣中总黄酮提取工艺条件。方法:以总黄酮得率为指标,采用单因素试验考察乙醇体积分数、提取时间、料液比、提取温度及回流次数5个影响因素对甜叶菊渣中总黄酮得率的影响;在单因素试验基础上,选取乙醇体积分数、提取时间、料液比、提取温度为考察因素,采用正交设计法优化甜叶菊渣中总黄酮的提取工艺条件。结果:各因素对甜叶菊渣中总黄酮得率的影响顺序为料液比>提取时间>乙醇体积分数>提取温度。最佳提取工艺条件为乙醇体积分数80%,提取时间2.5 h,料液比1∶20,提取温度80℃,回流3次,总黄酮得率4.21%。结论:该优选工艺简单易行、成本低廉、安全可靠,有利于甜叶菊资源的合理利用。Objective： To optimize extraction technology conditions of total flavonoids from residues of Stevia rebaudiana. Method： With yield of total flavonoids as index, effect of the concentration of ethanol, extraction time, solid-liquid ratio, extraction temperature and reflux extraction times on yield of total flavonoids from S. rebaudiana were studied by single-factor test; Basis on single factor test, the concentration of ethanol, extraction time, solid-liquid ratio, extraction temperature were chosen as factors, extraction technology of total flavonoids from residues of S. rebaudiana was optimized by orthogonal test. Result： Influence of various factors on yield of total flav0noids were in order of solid-liquid ratio 〉 extraction time 〉 the concentration of ethanol 〉 extraction temperature. Optimum extraction technology was： reflux extracted 3 times with 20 times the amount of 80% ethanol at 80℃, extraction time of 2.5 h, yield of total flavonoids of 4.21%. Conclusion： This optimized technology was simple, reliable and safe with low cost, and it benefited to rational utilization of S. rebaudiana resources.

关 键 词：甜叶菊 总黄酮 提取工艺 

分 类 号：R283.6[医药卫生—中药学]