近红外光谱法快速测定消渴丸干模丸中葛根素含量  

Rapid Determination of Puerarin in Dry Pills of Xiaoke Pills by Near-infrared Spectroscopy

作　　者：石猛 尹震 孙幸 梁智琳 王江超 雷婷 

机构地区：[1]广州中一药业有限公司,广州510530

出　　处：《中国实验方剂学杂志》2012年第22期88-90,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2009ZX09308-003)

摘　　要：目的:采用近红外光谱法对消渴丸干模丸中葛根素含量进行快速测定。方法:以HPLC分析值作为参照,采用近红外漫反射光谱技术采集消渴丸干模丸的近红外光谱,结合偏最小二乘法建立葛根素含量的快速测定方法,并对未知样品进行含量预测。结果:建立的葛根素校正模型R2=0.948 2,RMSECV=0.248,RPD=4.40。对验证集样品预测平均相对偏差为3.51%。结论:该方法准确、快速、简便,可作为消渴丸中间产品质量检测和实时在线质量控制的方法。Objective： To determine the content of puerarin in dry pills of Xiaoke pills rapidly by near- infrared spectroscopy. Method： Near-infrared diffuse reflectance spectroscopy technology was used to collect NIR spectra Of the dry pills. With HPLC analysis values as reference, the fast determination method for puerarin was established with partial least squares （PLS）. Result： R2, RMSECV and RPD of the calibration model for puerarin were 0. 948 2, 0. 248 and 4.40, respectively. The average relative deviation of the predication set was 3.51%. Conclusion： The method is accurate, fast and simple, which could be generalized to the quality monitor of intermediates and on-line quality control in the manufacture process of the Xiaoke pills.

关 键 词：近红外光谱法 消渴丸 葛根素 

分 类 号：R284.1[医药卫生—中药学]