产油藻株Scenedesmus sp胞外碳酸酐酶活性及光合作用活性的研究  

The study on extracellular carbonic anhydrase activity and photosynthesis activity of oil-producing alga Scenedesmus sp

作　　者：孙新健 罗生军[2] 范晓蕾[2] 杨志满 高岚[1] 郭荣波[2] 

机构地区：[1]兰州大学,甘肃兰州730000 [2]中国科学院青岛生物能源与过程研究所,山东青岛266101

出　　处：《可再生能源》2012年第11期99-103,共5页Renewable Energy Resources

基　　金：国家自然科学基金项目(31101918);山东省科技发展计划项目(2011GHY11531;2010GHY10504)

摘　　要：以产油藻株Scenedesmus sp为研究对象,利用两种CO2浓度分别为0.035%(空气)和2%培养Scenedesmus sp(栅藻)细胞。并对胞外碳酸酐酶(carbonic anhydrase,CA)活性进行测定,同时监测光合作用中的叶绿素荧光活性的变化。结果发现:在2%CO2培养情况下,检测栅藻细胞胞外碳酸酐酶无活性,光合系统叶绿素荧光活性及生物质积累没有受到CA抑制剂乙酰唑胺(AZ)的影响;0.035%(空气)培养下,胞外碳酸酐酶有较高活性,用AZ抑制胞外碳酸酐酶活性后,光合系统叶绿素荧光活性明显降低,生物质积累也受到抑制。试验结果说明,在较低浓度CO2培养的情况下,胞外碳酸酐酶对提高光合系统活性和光合作用效率具有重要的意义,可以有效促进生物质的积累。Two concentrations of CO2（0.035% and 2%） was used to culture Scenedesmus sp,extracellular carbonic anhydrase（CA） activity were determined and chlorophyll fluorescence activity changes were monitored.The results showed that： when in 2% CO2cultured,extracellular carbonic anhydrase activity of algal cells could not be determined,chlorophyll fluorescence of photosynthetic system activity and biomass accumulation also were no significant difference.In air cultured（0.035%）,high extracellular carbonic anhydrase activity was determined.With acetazolamide（AZ） to inhibite extracellular carbonic anhydrase activity,chlorophyll fluorescence activity and biomass accumulation had a obviously difference.The above data show extracellular carbonic anhydrase has a great significance to improve photosynthetic efficiency and efficient of biomass accumulation in the case of low concentrations of CO2.

关 键 词：栅藻 生物质 碳酸酐酶 叶绿素荧光活性 

分 类 号：TK6[动力工程及工程热物理—生物能] S216.2[农业科学—农业机械化工程]