野菊花药材HPLC指纹图谱  

HPLC Fingerprint of Chrysanthemum indicum

作　　者：林丽美[1,2] 李春[3] 刘塔斯[1] 许招懂 刘菊妍 廖端芳[1] 

机构地区：[1]湖南中医药大学,长沙410208 [2]广州星群(药业)股份有限公司,广州510288 [3]中国中医科学院中药研究所,北京100700 [4]广州医药集团有限公司,广州510130

出　　处：《中国实验方剂学杂志》2012年第22期120-123,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省科技厅项目(2011FJ7008);湖南省教育厅项目(11C0958);湖南省十二五重点学科药学学科项目

摘　　要：目的:建立野菊花药材的指纹图谱,为野菊花药材的质量控制提供依据。方法:采用HPLC,Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-1.0%醋酸水为流动相梯度洗脱,流速1.0 mL·min-1,检测波长290 nm,柱温30℃,进样10μL。结果:建立了16批野菊花药材的指纹图谱,选择11个野菊花样品作为标准药材,标准药材有16个共有峰,多数峰可以达到较好分离,具有较高的相似度。结论:建立的高效液相指纹图谱有较好的精密度、重复性和稳定性,可作为野菊花质量评价参考。of quality control Eclipse XDB-Cls Objective： To establish HPLC fingerprint and identification of the Chinese crude drug.（4.6mm×250mm,5μm） column with of C. indicum to provide the basis for the standards Method： The HPLC method was used on an Agilent acetonitrile-0. 1% acetic acid as mobile phase by gradient elution at the detection wavelength of 290 nm and the column temperature was set at 30℃ with the flow rate of 1.0 mL -min-1 ; the sample injection was 10 μL. Result： HPLC fingerprints of 16 samples of C. indicum were established. 16 common peaks were selected as the fingerprint peaks in 11 samples. Among the obtained fingerprints, most of the detected peaks were separated effectively. 11 samples had high similarities. Conclusion： The established HPLC fingerprint has desirable accuracy, repeatability and stability, which can be used for one of the quality control of C. indicum.

关 键 词：野菊花 高效液相色谱 指纹图谱 质量控制 

分 类 号：R284.1[医药卫生—中药学]