泛油对千金子主要有效成分含量的影响  

Impact of Oil-releasing to Content of Main Effective Components of Semen Euphorbiae

作　　者：张宏伟[1] 金锋[1] 张振凌[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第22期124-126,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业科研专项子项目(201107008)

摘　　要：目的:比较泛油对千金子种仁中脂肪油及千金子甾醇的含量的影响。方法:索式提取法提取脂肪油并测定含量;高效液相色谱法测定千金子甾醇含量,Agilent Rx-SIL液相色谱柱(4.6 mm×250 mm,5μm),流动相正己烷-乙酸乙酯-乙腈(87.5∶10∶2.5),柱温30℃,检测波长275 nm,体积流量1.0 mL·min-1。结果:未泛油千金子种仁脂肪油平均含量为62.48%,千金子甾醇平均含量为1.20%;泛油千金子种仁脂肪油平均含量为53.66%,千金子甾醇平均含量为1.10%。结论:千金子种仁泛油后其脂肪油及千金子甾醇含量均降低。Objective ： Compare the content of the fat oil and the euphorbia factor L1 of semen euphorbiae seed kernel before and after oil-releasing. Method： Soxhlet extraction was used to extract the fat oil and determine content. The content of euphorbia factor L1 were determined by HPLC. An Agilent rx-sil column （4.6mm×250mm,5μm） was used with normal hexane-ethyl acetate-acetonitrile （87.5： 10： 2.5） as the mobile phase, the flow velocity was at 1.0 mL-min-1, UV detection was at 275 nm and temperature was at 30℃. Result： The content of the fat oil and the euphorbia factor Li of normal semen euphorbiae seed kernel were 62.48% and 1.20%. The content of the fat oil and the euphorbia factor L1 of oil-releasing semen euphorbiae seed kernel was 53.66% and 1.10%. Conclusion： The content of the fat oil and the euphorbia factor L1 of oil-releasing semen euphorbiae seed kernel was reduced.

关 键 词：千金子 泛油 脂肪油 千金子甾醇 含量测定 

分 类 号：R284.1[医药卫生—中药学]