甘草甜味素中酪氨酸酶抑制剂的筛选  

Screening and Identification of Tyrosinase Inhibitors in Glycyrrhiza Extract by High Performance Liquid Chromatography

作　　者：邢珍珍[1,2] 孟庆艳 卢亚玲[1,2] 张红喜[2] 申雪花[2] 刘文杰[1,2] 

机构地区：[1]塔里木盆地生物资源保护与利用重点实验室,新疆阿拉尔843300 [2]塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《中国实验方剂学杂志》2012年第22期163-166,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：兵团青年科技创新资金专项(2012CB028);新疆生产建设兵团科技攻关项目(2011AB030)

摘　　要：目的:从生产甘草酸的副产品甘草甜味素中寻找酪氨酸酶抑制剂。方法:采用高效液相色谱与酶抑制分析相结合,利用电喷雾MS-MS串联质谱方法及紫外光谱对活性成分进行结构确认,建立了甘草甜味素中酪氨酸酶抑制活性成分快速筛选与鉴别方法。HPLC色谱柱分离后,利用二极管阵列检测器得到各活性组分的紫外光谱图,流出液与在96孔板中与酪氨酸酶、L-多巴作用,利用酶标仪检测酶剩余活力,从而实现筛选的目的。结果:利用此方法从甘草甜味素中筛选出4个具有明显酪氨酸酶抑制活性的化合物,通过ESI-MS/MS分析和紫外光谱图,结合相关文献,实现了各化合物的快速鉴别。结论:4个化合物均为已知化合物,分别为甘草苷、18α-羟基甘草次酸甲酯、甘草查耳酮C和18α-甘草次酸。Objective： A new method based on the HPLC scaveng enzyme inhibition activity and HPLC MS/MS method for the identification of active constituent from glycyrrhiza extract was developed. Method： Glycyrrhiza extract was separated by HPLC and the outlet of HPLC photo diode array detector flow cell was receiving every 30s. Enzyme inhibition react with tyrosinase in post column reaction and IMARK detect at 419 nm. Result and Conclusion： The results showed that 4 compounds in glycyrrhiza extract have scavenging enzyme inhibition activity, and all the 4 compounds including Liquiritin, methyl 18a-hydroxyglycyrrhetate, licochalcone C, 18a-glycyrrhetic acid were identified by MS/MS spectrogram and UV spectrum.

关 键 词：甘草甜味素 高效液相色谱 筛选 酪氨酸酶 

分 类 号：R284.1[医药卫生—中药学]