南蛇藤总萜对MGC-803细胞侵袭和迁移能力的影响  

Effect of Celastrus orbiculatus Extract on the Invasion and Migration in Human Gastric Carcinoma MGC-803 Cells

作　　者：朱耀东[1] 钱亚云[1] 马慧[1] 郭试瑜[2] 久光正[2] 刘延庆[1] 

机构地区：[1]扬州大学医学院中西医结合临床医学系肿瘤研究室,江苏扬州225009 [2]昭和大学医学部生理学教研室

出　　处：《中国实验方剂学杂志》2012年第22期180-183,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173603);江苏省自然科学基金项目(BK2012686);江苏省中医药科技项目(LZ11210)

摘　　要：目的:探讨南蛇藤总萜对人胃癌MGC-803细胞侵袭和迁移能力的影响,并对其机制进行初步探讨。方法:体外培养MGC-803细胞,分为南蛇藤总萜组(质量浓度分别为20,40,80 mg·L-1)、阴性对照组和阳性对照组,采用MTT法检测南蛇藤总萜对MGC-803细胞增殖的抑制作用;采用Transwell小室法检测南蛇藤总萜处理MGC-803细胞24 h后,细胞侵袭和迁移能力的改变;Western blotting法检测处理24 h后基质金属蛋白酶(MMP)-2和MMP-9的表达情况。结果:南蛇藤总萜能抑制MGC-803细胞的增殖,呈一定的浓度及时间依赖性;南蛇藤总萜40,80 mg·L-1能显著降低MGC-803细胞的穿膜细胞数(P<0.01),并呈浓度依赖性;南蛇藤总萜能降低MGC-803细胞中MMP-2和MMP-9蛋白的表达水平,并有浓度依赖性。结论:南蛇藤总萜能抑制人胃癌MGC-803细胞的侵袭和迁移能力,其机制可能与抑制MMP-2,MMP-9的表达有关。Objective ： To study the effect and mechanism of Celastrus orbiculatus ThuMb. extract （COE） on invasion and migration of human gastric carcinoma MGC-803 ceils. Method： The human gastric carcinoma MGC-803 ceils were divided into negative and positive control group and COE groups （20, 40, 80 mg.L-1, respectively）. The cytotoxicity effect was measured by using the MTT assay. The invasion and migration ability of MGC-803 were assayed by transwell assay. The expression levels of matrix metalloproteinase （MMP） -2 and MMP-9 in MGC-803 were examined by western blot assay. Result： COE suppressed the proliferation of MGC-803 cells in a time and does-dependent manner. The concentrations of 40, 80 mg.L-1 of COE significantly inhibited the invasion and migration activity of MGC-803 cells. In addition, the expression levels of MMP-2, MMP-9 were dramatically suppressed by COE in a concentration-dependent manner. Conclusion： COE could obviously inhibit the migration and invasion ability of MGC-803 cells. Its mechanism may be related to the down-regulated expression of MMP-2 and MMP-9.

关 键 词：南蛇藤提取物 侵袭 迁移 基质金属蛋白酶-2 基质金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]