毛鸡骨草含药血清体外抗乙型肝炎病毒作用的研究  

Inhibitory Effect of Serum Containing Abrus mollis on Hepatitis B Virus in vitro

作　　者：陈晓白[1] 王晓平[1] 赵仕花[1] 

机构地区：[1]玉林师范学院生命科学与技术学院,广西玉林537000

出　　处：《中国实验方剂学杂志》2012年第22期218-221,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西教育厅科研项目(200710MS052);广西玉林市科技局项目(玉市科攻0881038)

摘　　要：目的:观察毛鸡骨草(Abrus mollis)含药血清体外抗乙型肝炎病毒(HBV)的作用。方法:采取中药血清药理实验方法,以HepG2.2.15细胞为研究模型,将毛鸡骨草含药血清加入HepG2.2.15细胞培养液中培养,分别在72 h和144 h收集细胞培养上清液,采用酶联免疫吸附实验法(ELISA法)和荧光定量PCR(FQ-PCR)法检测毛鸡骨草含药血清对HepG2.2.15细胞株表达的乙型肝炎表面抗原(HBsAg)和HBV DNA复制的影响。结果:毛鸡骨草含药血清对HepG2.2.15细胞上清液中HBsAg的表达和HBV DNA复制都有不同程度的抑制,以给药剂量为30 g·kg-1·d-1的毛鸡骨草含药血清组作用144 h对HBsAg的抑制率和对HBV DNA复制的抑制率最明显,抑制率分别为34.4%和41.7%。结论:毛鸡骨草在体外有一定的抗HBV作用。Objective： To observe the inhibitive effect of the serum containing Abrus mollis on hepatitis B virus （HBV）. Method： HepG2. 2. 15 cells were cultured in medium containing the serum with A. mollis for 72 h and 144 h. The Effect of the serum containing A. mollis on HBsAg and HBV DNA reproduction in culture media of the HepG2.2.15 cells were determined by ELISA and fluorescence quantitative pdymerase chain reaction （FQ- PCR）. Result： The serum containing A. mollis had a inhibiting effect on the HBsAg and HBV DNA reproduction with maximum inhibition ratio adding up to 34.4% and 41.7% respectively in which the affective time is 144 h and the density is 30 g-kg-1.d-1. Conclusion： A. mollis can significantly inhibit HBV in vitro.

关 键 词：毛鸡骨草含药血清 HepG2-2-15细胞 乙型肝炎病毒 

分 类 号：R285.5[医药卫生—中药学]