脑尔康及其拆方组对阿尔茨海默病小鼠模型脑内BDNF表达的影响  

Influences of Naoerkang and its Splited Recipes on Expression of BDNF in the Brains of AD Mice Model

作　　者：马新欣[1] 李玺[1] 侯吉星[2] 袁海峰[1] 王娟[3] 

机构地区：[1]西安交通大学医学院第二附属医院,西安710004 [2]西安市精神卫生中心,西安710061 [3]第四军医大学西京医院检验中心,西安710032

出　　处：《中国实验方剂学杂志》2012年第22期227-232,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省科技攻关计划项目[2005K10-G12]

摘　　要：目的:观察复方中药脑尔康及其拆方组对阿尔茨海默病(Alzheimer’s disease,AD)模型小鼠不同脑区脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响。方法:随机将70只昆明小鼠分为7组,每组10只,采用ip氯化铝(AlCl3)溶液100 mg·kg-1ip,隔日1次共40 d,建立AD模型小鼠,于学习记忆成绩测试后,空白组和模型组每天给予生理盐水ig(0.5 mL·d-1);全方组及各拆方组每天分别给予相应药物ig(14.3 g·kg-1·d-1),连续30 d。测定BDNF在各组小鼠右侧皮层、海马、齿状回的阳性表达。结果:①各用药组与模型组比较,全方组及拆方1号组BDNF阳性反应物质表达明显增多(P<0.01);拆方2,3,4号组BDNF表达仅不同程度增多(P<0.05);②各拆方组分别与全方组比较,拆方1号组与全方组BDNF阳性反应物质表达无明显差异;③拆方2,3,4号组与拆方1号组间比较,拆方2号组仅在DG,Cortex区域表达的平均灰度值与拆方1号无明显差异;拆方3,4号组仅在DG区域表达的平均灰度值与拆方1号无明显差异。结论:拆方1号组及脑尔康全方组促进AD模型小鼠脑内BDNF表达作用明显优于其他拆方组,两者疗效相当,但前者药味精简。Objective： To observe the amelioration of Naoerkang and its splited prescription on the expression of brain-derived neurotrophic factor （BDNF） in cortex and hippocampus of senile dementia mice model. Method： Seventy male Kunming mice were randomly divided into 7 groups with 10 for each group, then ip A1C13 solution 100 mg.kg-1 , ip, q. o. d for 40 days to induce the model. After the examination of their ability in learning and memory, each mouse of the control group and model group had been forced to drink saline solution （0.5 mL .d-l）, the other groups had been forced to drink the response medicine （14.3 g .kg-1.d-1） every day for 30 days. The positive expression of BDNF in right cortex, hippocampus, and the dentate gyrus of the mice in each group were examined. Result： ①Compared with the model group, the expression of BDNF was increased evidently （P 〈0.01） in the mice of Naoerkang group and splited prescription 1 group, while the expression of different levels increased only in other groups （P 〈 0. 05 ）. ②All of its decomposed recipes compared with the Naoerkang group, there were no significant differences existed in splited prescription 1 and Naoerkang group. ③Compared with the splited prescription 1, there were no significant differences existed neither in dentate gyrus and cortex of splited prescription 2, nor in dentate gyrus of splited prescription 3 and 4. Conclusion： Decomposed recipe 1 and Naoerkang groups have a better effect of promoting the expression of BDNF in brains of AD mice model than any other splited prescription.

关 键 词：脑尔康 拆方实验研究 阿尔茨海默病 脑源性神经营养因子 

分 类 号：R285.5[医药卫生—中药学]