黄连阿胶汤对对氯苯丙氨酸致失眠模型小鼠神经递质的影响  

Effect of Huanglian Ejiao Decoction to Neurotransmitter of p-chlorophenylalanine Induced Insomnia Mice

作　　者：贾利利[1] 周宁[1] 李凯[1] 张振凌[1] 

机构地区：[1]河南中医学院药学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第22期240-242,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2010年中医药行业科研专项项目(201007010);河南中医学院博士基金项目(BSJJ2010-06)

摘　　要：目的:研究黄连阿胶汤对对氯苯丙氨酸(PCPA)致失眠模型小鼠的神经递质5-羟色胺(5-HT)及γ-氨基丁酸含量的影响,分析黄连阿胶汤对失眠的作用。方法:空白组、模型组给予生理盐水溶液;阳性组给予天王补心混悬液1.5 g·kg-1;给药组给予黄连阿胶汤混悬液5 g·kg-1;给药体积均为10 mL·kg-1,给药1周后停止灌胃给药。之后阳性组、给药组、模型组腹腔注射PCPA 350 mg·kg-1,给药体积为20 mL·kg-1,空白组腹腔注射同体积的生理盐水溶液,连续2 d。采用酶免法测定5-HT及γ-氨基丁酸含量。结果:黄连阿胶汤组、空白组、模型组、天王补心混悬液组5-HT分别为(89.12±5.81),(116.08±8.36),(68.45±17.63),(98.08±4.11)pg.mg-1,γ-氨基丁酸分别为(13.72±1.83),(12.01±0.66),(16.13±2.26),(14.17±2.17)μmol·L-1。给药组与空白组、模型组相比,5-HT和γ-氨基丁酸含量均具有显著性差异;给药组与阳性组相比,5-HT有显著性差异,而γ-氨基丁酸没有显著性差异。结论:黄连阿胶汤可逆转由PCPA引起的失眠,改善脑内神经递质5-HT的含量,为黄连阿胶汤治疗失眠提供了实验依据。Objective： To study the effect of Huanglian Ejiao decoction on serotonin （5-HT） and γ- aminobutyric acid content of p-chlorophenylatanine （PCPA） induced insomnia model in mice. Method： Normal group and model group were given saline solution; positive group was given Tianwang Buxin suspension 1.5 g .kg-1 ; medicine group was given Huanglian Ejiao decoction 5 g .kg-1 ; dosing volume was 10 mL .kg-1 and cycle was one week. Then positive, medicine and model groups were intraperitoneally injected PCPA 350 mg .kg-1 with dosing volume of 20 mL.kg-1, and normal group was given same volume of saline solution all for 2 days. The contents of 5-HT and γ-aminobutyric acid were determined by enzyme immunoassay. Result： The contents of 5-HT and γ-aminobutyric acid in medicine, normal, model and positive group were （89.12±5.81 ） pg . mg-1, （13.72±1.83） μmol.L-1; （116.08±8.36） pg.mg-1 （12.01 ±0.66） μmol.L-1 （68.45±17.63） pg.mg-1（16. 13 ±2.26） μmol＇L-1 -1 , ; （98.08 ±4.11） pg.mg （14. 17±2. 17） μmol . Compared with normal group and model group, 5-HT and γ-aminobutyric acid content in treatment groups showed significant differences. Compared with positive group, only 5-HT content of treatment groupindicated significant difference. Conclusion： Huanglian Ejiao decoction could reverse PCPA induced insomnia, improve the content of neurotransmitters in the brain, and provide experimental basis for the treatment of insomnia by Huanglian Ejiao decoction.

关 键 词：黄连阿胶汤 对氯苯丙氨酸 失眠 5-羟色胺 Γ-氨基丁酸 

分 类 号：R285.5[医药卫生—中药学]