沙漠绢蒿黄酮抑制肺癌A-549细胞增殖  

Inhibiting Effects of Flavonoids from Seriphidium santolium on Human Lung Carcinoma Cell Line A-549

作　　者：张成军[1] 邓雁如[2] 王颖[2] 牟佳佳[2] 

机构地区：[1]天津中医药大学第二附属医院,天津300150 [2]天津中医药大学,天津市中药化学与分析重点实验室,天津300193

出　　处：《中国实验方剂学杂志》2012年第22期243-247,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究从沙漠绢蒿中得到的黄酮类化合物体外对肺癌A-549细胞增殖的影响。方法:利用硅胶柱色谱等方法分离纯化6种黄酮类化合物,分别为5,7,4'-三羟基-6-甲氧基黄酮、5,7-二羟基-6,3',4',5'-四甲氧基黄酮、5,7,4'-三羟基-6,3',5'-三甲氧基黄酮、5,7-二羟基-6,3',4'-三甲氧基黄酮、槲皮万寿菊素-3,6-二甲醚-7-O-β-D-葡萄糖苷、木犀草素-7-O-β-D-葡萄糖苷-(3'-O-7″)-槲皮素-3″-甲醚;采用MTT法测定6种黄酮类化合物对肺癌A-549细胞增殖的抑制作用;采用流式细胞仪检测细胞周期及细胞凋亡。结果:受试的6种黄酮类化合物中的5种化合物对肺癌A-549细胞增殖具有明显抑制作用,其抑制率与化合物浓度呈一定的相关性;流式细胞仪检测发现其中2种化合物(5,7,4'-三羟基-6-甲氧基黄酮和5,7,4'-三羟基-6,3',5'-三甲氧基黄酮)使细胞周期分别阻滞于G1和G2期,另1种化合物(5,7-二羟基-6,3',4'-三甲氧基黄酮)可通过诱导细胞凋亡及细胞周期阻滞抑制A-549细胞增殖。结论:沙漠绢蒿黄酮对人肺癌细胞株A-549增殖具有明显抑制作用,使细胞发生周期阻滞或凋亡。proliferation of the on silica gel. Objective： To study the inhibitory effect of flavonoids from Seriphidium santolium on the lung carcinoma A549 cell line. Method： Six flavonoids were isolated by column chromatography They were identified as hispidulin, arteanoflavone, 5, 7, 4＇-trihydroxy-6, 3＇, 5＇- trimethoxyflavone, eupatilin, quercetagetin-3, 6-dimethyl ether-7-O-β-D-glucoside, luteolin-7-O-β-D-glucoside- （3＇-0-7＂） -quercetin-3＂-methyl ether. MTT was performed to examine the proliferation of A-549 cells, and flow cytometry was used to determine the cell cycle distribution and cell apoptosis. Result： There was a dose-dependent inhibition of cell proliferation of A-549 by five compounds. The antiproliferative effect of compound l （hispidulin） was attributed to its inducing cell cycle （G1 ） arrest, and compound HI （5, 7, 4＇-trihydroxy-6, 3＇, 5＇-trimethoxyflavone） attributed to its inducing cell cycle （G2） arrest. Compound IV （eupatilin） induced the apoptosis and inhibited the cell circle of A-549 cell. Conclusion： Flavonoids from S. santolium could exert an anti-lung cancer effect via induction of cell cycle arrest or cell apoptosis.

关 键 词：沙漠绢蒿 黄酮类 人肺癌A-549细胞 增殖 细胞周期 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]