加味四君固本汤与Iressa联合用药对裸鼠移植瘤的影响  

Effect of Jiawei Sijun Guben Decoction with Iressa on Human Non Small Cell Lung Cancer Cell A549 Xenograft Model

作　　者：朱成全[1] 曾伟霞 肖烈钢[1] 何本夫[1] 

机构地区：[1]中国人民解放军第四二一医院中西医结合科,广州510318 [2]广州朗日生物技术有限公司,广州510663

出　　处：《中国实验方剂学杂志》2012年第22期263-266,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：全军医学科学技术研究"十一五"计划课题(06MA124)

摘　　要：目的:研究加味四君固本汤对皮下成瘤裸鼠的影响。方法:使用人非小细胞肺癌A549细胞在裸鼠皮下接种成瘤,随机分成对照组,易瑞沙(Iressa)治疗组(100 mg·kg-1·d-1),中药治疗组(加味四君固本汤0.10 g·kg-1·d-1),加味四君固本汤和Iressa联合治疗组,观察肿瘤生长情况及裸鼠生存质量,并采用免疫印记实验(Western blot)和实时荧光定量PCR(RT-PCR)检测各组肿瘤中上皮生长因子受体(EGFR)蛋白质与mRNA的表达情况。结果:西药组、中药组和联合用药组的肿瘤抑制率分别为55.75%、35.22%和82.3%,与对照组比较差异显著(P<0.01),联合用药组与单独用药组差异具有统计学意义(P<0.01)。观察治疗期间裸鼠的运动量、饮食、体重等指标,显示联合用药组裸鼠的生存质量优于西药组。Western Blot与RT-PCR结果显示联合用药组的肿瘤组织中增殖相关的指标明显低于其他3组。结论:加味四君固本汤与Iressa联合用药能更好地抑制肿瘤生长,对肿瘤的治疗效果明显优于Iressa组。加味四君固本汤能提高裸鼠治疗过程中的生存质量。Objective： To study the effect of Jiawei Sijun Guben Decoction with iressa on human non small cell lung cancer cell A549 xenograft model. Method： Subcutaneous A549 xenograft nudemice were divided into 4 groups randomly： control group （treated with glucose solution）, Western medicine treatmen （WMT） group （Iressa, 100 mg .kg-1.d-1）, Chinese medicine treatment （CMT） group （Jiawei Sijun Guben decoction, 0.10 g-kg-1.d-1） and Combined treatment （CT） group （Jiawei Sijun Guben decoction ＋ Iressa）. Tumor growth and life quality of tumor-bearing nude mice were observed. Western blotting and RT-PCR were used to detect the mRNA and protein expression of EGFR. Result： The tumor volumes of WMT, CMT and CT groups decreased greatly compared with control group, the inhibitory rate of tumor of was 55.75% , 35.22% and 82.3% respectively （ P 〈 0.01 ）. And the significant difference of tumor volumes in CT group was also showed compared with WMT and CMT group respectively （P 〈 0.01 ）. Life quality （amount of exercise, diet and body weight） of CT group was better than that of WMT group. EFGR protein and mRNA expression in tumor tissue of CT group were significantly lower than those of other groups. Conclusion： Jiawei Sijun Guben decoction plus Iressa therapy could inhibit tumor growth more effectively, which led to better treatment effect instead of using Iressa treatment alone. Jiawei Sijun Guben decoction therapy could greatly improve life quality of tumor-bearing nude mice during the treatment.

关 键 词：加味四君固本汤 裸鼠 移植瘤 生存质量 非小细胞肺癌 

分 类 号：R285.5[医药卫生—中药学]