疏风凉血补肾方对ITP模型小鼠免疫调控的影响  

Immune Regulation of Shufeng Liangxue Decoction on Immune Thrombocytopenic Purpura Model in Mice

作　　者：唐志宇[1] 陈志雄[2] 于天启[2] 

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第三临床医学院,广州510405

出　　处：《中国实验方剂学杂志》2012年第22期279-282,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察疏风凉血补肾方对免疫性血小板减少性紫癜(ITP)模型小鼠外周血血小板(PLT),干扰素γ(IFN-γ),CD80,CD86及程序性细胞死亡基因(PD-1)的表达变化的影响。方法:制备豚鼠抗小鼠血小板血清(APS),连续注射小鼠,复制小鼠ITP模型,疏风凉血补肾方高、中、低剂量灌胃给药(112.5,75,37.5 g·kg-1·d-1),强的松组灌胃强的松(10 mg·kg-1·d-1),对照组、模型组给予生理盐水,连续给药7 d。应用血细胞分析仪测定PLT,酶联免疫吸附试验(ELISA)检测细胞因子IFN-γ,流式细胞仪检测外周血淋巴细胞CD80,CD86及PD-1的表达。结果:模型小鼠外周血PLT数持续减低,IFN-γ,CD80,CD86及PD-1明显增高,给予疏风凉血补肾方治疗后模型小鼠外周血PLT数明显增高(P<0.05),IFN-γ,CD80,CD86,PD-1明显降低(P<0.05)。结论:疏风凉血补肾方能升高ITP模型小鼠PLT,降低IFN-γ,CD80,CD86及PD-1的表达,改善细胞免疫的失衡,达到治疗ITP的作用。Objective： To observe the impact influence of Shufeng Liangxue decoction of on platelet count, interferon-~/ （IFN-γ） , CD80, CD86 and programmed cell death gene （PD-1） of the model mousein of peripheral blood in disease of immune thrombocytopenic purpura （ITP） model mice in peripheral blood on treatment with Shufeng Liangxue decoction. Method： It was designed to use guinea pig anti-mouse guinea pig platelet serum （APS） was injected into mice continuously in order to induce the immune thrombocytopenic purpura mouse models. Then mice of Shufeng Liangxue treatment groups, positive group, model group and control group were administered orally with Shufeng Liangxue decoction （ 112.5, 75, 37.5 g.kg-1.d-1 ） of large, medium and small doses prednisone （10 mg .1g-l.d-1） and saline respectively, and all groups were maintained its treatment for eontinuel 7 days. The blood cell counter was used to detect platelet count, The the levels of cytokine IFN-γ was detected by enzyme linked immunosorbentassay （ELISA）, CD80, CD86 and PD-1 were detected by flow cytometry. Result： Platelet count reduced and IFN-γ, TCD80, CD86 and PD-1 increased significantly in model group mice. Platelet count increased significantly （P 〈0.05） and IFN-γ, TCD80, CD86 and PD-1 significantly decreased （P 〈 0.05） in Shufeng Liangxue decoction group mice. Conclusion： The Shufeng Liangxue decoction could enhance platelet count decrease IFN-γ, CD80, CD86 and PD-lexpress, improve unbalance of cellular immunity function in ITP model miceto to treat ITP.

关 键 词：疏风凉血补肾方 免疫性血小板减少性紫癜 协同刺激分子 细胞因子 免疫调控 

分 类 号：R285.5[医药卫生—中药学]