TRFIA与ELISA法检测乙肝病毒血清学标志物的分析与比较  

Analysis and Comparison of HBV Serum Marker Results between TRFIA and ELISA

作　　者：吴宗华[1] 

机构地区：[1]中山大学附属第三医院检验科,广东广州510630

出　　处：《临床医学工程》2012年第11期1967-1968,共2页Clinical Medicine & Engineering

摘　　要：目的比较分析时间分辨荧光免疫法(TRFIA)、酶联免疫吸附法(ELISA)对乙型肝炎病毒感染血清学标志物(HBV-M)的检测结果。方法采用TRFIA和ELISA法对182例肝病就诊患者同时进行HBV-M检测,两者结果不符合者双孔复查。结果 TRFIA法测定乙肝表面抗原(HBsAg)、乙肝e抗原(HBeAg)、乙肝e抗体(HBeAb)灵敏度高于ELISA法,结果符合率分别为98.9%、96.7%、99.5%、90.7%、88.5%。经配对卡方检验,两种方法测定HBsAg,HBeAg,HBeAb的结果比较无显著性差异(P>0.05),而测定乙肝表面抗体(HBsAb)、乙肝核心抗体(HbcAb)的结果比较有显著性差异(P<0.05)。乙肝病毒血清学标志物的医学模式比较中,HBsAb与HBsAb、HBcAb阳性模式有显著性差异(P<0.05),其它医学模式无显著性差异。结论 TRFIA法测定HBV-M是一种灵敏度高,结果符合率高的定量检测技术,对提高临床检测水平、指导临床治疗、监测方面有实用价值。Objective To compare and analyze TP, FIA with ELISA in the detection results of hepatitis B virus serum marker （HBV-M）. Methods TRFIA and ELISA were applied respectively to detect serum HBV markers for 182 cases of serum samples. Both the results did not accord with double holes review. Results TR.FIA filnctioned better than ELISA in detecting HBsAg, HBeAg and HBeAb for its high sensitivity. The coincidence rates of positive HbsAg, HbsAb, HBeAg, HBeAb and HBcAb were 98.9%, 96.7%, 99.5%, 90.7% and 88.5% respectively. No remarkable significance had in HBsAg, HBeAg and HBeAb by paired Chi-square test （P 〉0.05）. In contrast, HBeAb and HBcAb were significant （P 〈0.05）. In comparison of HBV-M medical model, HBsAb and HBsAb-HBcAb models had significantly difference （P 〈0.05）, others had non-significance. Conclusions TRFIA method to detect HBV-M is a quantitative detection technique with high sensitivity, high coincidence rates, which can be used to increase diagnosis level and direct clinical treatment.

关 键 词：时间分辨荧光免疫分析技术 酶联免疫吸附试验 乙型肝炎病毒血清标志 

分 类 号：R392-33[医药卫生—免疫学]