用Nested-PCR诊断鸡弯曲菌病的研究  

Study on the Detection of Avian Campylobacteriasis by Nested Polymerase China Reaction(Nested-PCR)

作　　者：张康[1,2] 龚振华[1] 李蕾[1] 王洪伟 李玉清[1] 张丽娟[1] 王丽萍[1] 庄青叶[1] 于建敏[1] 李金平[1] 刘国庆 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]青岛易邦生物股份有限公司,山东青岛266032 [3]诸城市畜牧兽医管理局,山东诸城262200 [4]胶南市兴牧科技服务中心,山东胶南266400

出　　处：《中国动物检疫》2012年第11期44-46,共3页China Animal Health Inspection

基　　金：农业部公益性科技专项

摘　　要：本研究采用Nested-PCR方法,对于临床采集的感染空肠弯曲菌的病鸡组织样品进行了检测,研究结果表明,采用Nested-PCR方法检测空肠弯曲菌具有结果清晰,操作简便,快速等特点。研究还发现,空肠弯曲菌对鸡的致病性可能增强,应引起重视。The Nested-PCR was used in the study for the detection of Campylobacter jujuni in tissue samples from chickens clinically infected with Campylobacter jujunei.Results indicated that the Nested-PCR for C.jujunei was easy and rapid in operation with clear results.And it was also found that the pathogenicity of the bacterium was increased in chickens, which shond arouse much attention.

关 键 词：空肠弯曲菌 Nested PCR 禽病诊断 

分 类 号：S852.613[农业科学—基础兽医学] S858.31[农业科学—兽医学]