肝脏缺血再灌注损伤与一氧化氮和内皮素平衡关系的研究  

The study of relationship between hepatic ischemia/reperfusion injury and nitric oxide/endothelins balance

作　　者：吕平[1] 陈道达[1] 田源[2] 张景辉[2] 吴毅华 

机构地区：[1]同济医科大学附属协和医院普外科,武汉430022 [2]同济医科大学附属协和医院普外科实验室,武汉430022

出　　处：《中华实验外科杂志》2000年第2期161-162,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　研究一氧化氮 (NO)与内皮素 (ET)平衡关系 (NO/ET)的变化与肝脏缺血再灌注(I/R)损伤的关系。方法　采用鼠肝I/R模型 ,并应用不同工具药物观察血NO/ET比值的变化以及肝损伤的情况。结果　I/R急性期血NO/ET比值降低 (1.5 8± 0 .2 0至 0 .2 9± 0 .0 5 ,P <0 .0 1) ,肝损伤加重。NO供体L 精氨酸 (L Arg)与ET受体拮抗剂TAK 0 44在一定程度上可减轻肝I/R损伤 ,而NO合酶抑制剂L NAME进一步加重了损伤。结论　肝I/R损伤与NO/ET平衡关系破坏有关 ,调节其平衡关系可影响I/R损伤的程度。Objective To study the relationship between the hepatic ischemia/reperfusion (I/R) injury and the balance of nitric oxide/endothelins (NO/ET).Methods The changes of the ratio of NO/ET and the hepatic injury were studied in a rat hepatic I/R model with several tool drugs used.Results In the acute phase of hepatic I/R,the ratio of NO/ET in the blood was reduced (1.58±0.20 to 0.29±0.05, P<0.01 )and the hepatic damage was deteriorated. NO donor L Arg and ET receptor antagonist TAK 044 could alleviate the hepatic I/R injury in some degree, whereas NO synthase inhibitor L NAME aggravated the damage.Conclusion The hepatic I/R injury might be concerned with the disturbance of the balance relationship of NO/ET. Regulation of this balance relationship might have an effect on the I/R injury.

关 键 词：一氧化氮 内皮素 肝缺血 再灌注损伤 

分 类 号：R602[医药卫生—外科学]