Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells  

作　　者：LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing 

机构地区：[1]State Key Laboratory of Robotics,Shenyang Institute of Automation,Chinese Academy of Sciences,Shenyang 110016,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Department of Mechamcal and Btomedzcal Engineering,City University of Hong Kong,Hong Kong,China [4]Department of Lymphoma,Affiliated Hospital of Military Medical Academy of Sciences,Beijing 100071,China [5]State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China

出　　处：《Science China(Life Sciences)》2012年第11期968-973,共6页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos. 60904095 and 61175103);CAS FEA International Partnership Program for Creative Research Teams and the State Key Laboratory of Drug Research

摘　　要：Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties of single living cells under physiological conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells (RBCs) and three types of aggressive cancer cells (Burkitt's lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562). The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a microsphere glued onto a tip-less cantilever. The diameters of RBCs are significantly smaller than those of the cancer cells, and mechanical measurements indicated that Young's modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young's modulus than that of indolent cancer cells, which may improve our understanding of metastasis.Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy （AFM） enables researchers to measure mechanical properties of single living cells under physiologi- cal conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells （RBCs） and three types of aggressive cancer cells （Burkitt＇s lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562）. The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a micro-sphere glued onto a tip-less cantilever. The diameters of RBCs are sig- nificantly smaller than those of the cancer cells, and mechanical measurements indicated that Young＇s modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young＇s modulus than that of indolent cancer cells, which may improve our understanding of metastasis.

关 键 词：atomic force microscopy red blood cell cancer cell mechanical properties Young＇s modulus 

分 类 号：Q279[生物学—细胞生物学] TH742[机械工程—光学工程]