Sphingosine-1-phosphate (S1P) mediates darkness-induced stomatal closure through raising cytosol pH and hydrogen peroxide (H_2O_2) levels in guard cells in Vicia faba  

作　　者：MA YinLi SHE XiaoPing YANG ShuShen 

机构地区：[1]School of Life Sciences, Shaanxi Normal University, Xi'an 710062, China [2]School of Life Sciences, Shanxi Normal University, Linfen 041004, China [3]School of Life Sciences, Northwest A & F University, Yangling 712100, China

出　　处：《Science China(Life Sciences)》2012年第11期974-983,共10页中国科学（生命科学英文版）

基　　金：supported by the Graduate Education Innovation Program Projects of Ministry of Education, China (Grant No. F-0922)

摘　　要：The role and signaling of sphingosine-l-phosphate （SIP） during darkness-induced stomatal closure were examined in Vicia faba. Darkness substantially raised S 1P and hydrogen peroxide （H202） levels and closed stomata. These darkness effects were significantly suppressed by DL-threo-dihydrosphingosine （DL-threo-DHS） and N,N-dimethylsphingosine （DMS）, two inhibi- tors of long-chain base kinases. Exogenous SIP led to stomatal closure and H202 production, and the effects of SIP were largely prevented by the H202 modulators ascorbic acid, catalase, and diphenyleneiodonium. These results indicated that SIP mediated darkness-induced stomatal closure by triggering H202 production. In addition, DL-threo-DHS and DMS significantly suppressed both darkness-induced cytosolic alkalization in guard cells and stomatal closure. Exogenous SIP caused cytosolic alkalization and stomatal closure, which could be largely abolished by butyric acid. These results demonstrated that SIP syn-thesis was necessary for cytosolic alkalization during stomatal closure caused by darkness. Furthermore, together with the data described above, inhibition of darkness-induced H202 production by butyric acid revealed that S 1P synthesis-induced cytosolic alkalization was a prerequisite for H202 production during stomatal closure caused by darkness, a conclusion supported by the facts that the pH increase caused by exogenous SIP had a shorter lag and peaked faster than H202 levels and that butyric acid prevented exogenous SIP-induced H202 production. Altogether, our data suggested that darkness induced SIP synthesis, causing cytosolic alkalization and subsequent H202 production, finally leading to stomatal closure.The role and signaling of sphingosine-1-phosphate (S1P) during darkness-induced stomatal closure were examined in Vicia faba. Darkness substantially raised S1P and hydrogen peroxide (H 2 O 2 ) levels and closed stomata. These darkness effects were significantly suppressed by DL-threo-dihydrosphingosine (DL-threo-DHS) and N,N-dimethylsphingosine (DMS), two inhibitors of long-chain base kinases. Exogenous S1P led to stomatal closure and H 2 O 2 production, and the effects of S1P were largely prevented by the H 2 O 2 modulators ascorbic acid, catalase, and diphenyleneiodonium. These results indicated that S1P mediated darkness-induced stomatal closure by triggering H 2 O 2 production. In addition, DL-threo-DHS and DMS significantly suppressed both darkness-induced cytosolic alkalization in guard cells and stomatal closure. Exogenous S1P caused cytosolic alkalization and stomatal closure, which could be largely abolished by butyric acid. These results demonstrated that S1P syn-thesis was necessary for cytosolic alkalization during stomatal closure caused by darkness. Furthermore, together with the data described above, inhibition of darkness-induced H 2 O 2 production by butyric acid revealed that S1P synthesis-induced cytosolic alkalization was a prerequisite for H 2 O 2 production during stomatal closure caused by darkness, a conclusion supported by the facts that the pH increase caused by exogenous S1P had a shorter lag and peaked faster than H 2 O 2 levels and that butyric acid prevented exogenous S1P-induced H 2 O 2 production. Altogether, our data suggested that darkness induced S1P synthesis, causing cytosolic alkalization and subsequent H 2 O 2 production, finally leading to stomatal closure.

关 键 词：sphingosine-l-phosphate cytosol pH hydrogen peroxide DARKNESS stomatal closure 

分 类 号：Q554.6[生物学—生物化学] Q945.172