地高辛素标记探针检测HFRS病毒基因研究  

STUDY ON DETECTION OF HEMORRHAGIC FEVER WITH RENAL SYDNROME VIRUS GENE BY DIG LABELED PROBE

作　　者：朱进[1] 张云[1] 郭恒彬[1] 唐家琪[1] 吴光华[1] 

机构地区：[1]南京军区军事医学研究所,南京210002

出　　处：《中国人兽共患病杂志》2000年第2期13-15,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金

摘　　要：本实验用聚合酶链反应与随机引物标记法制备地高辛素标记的肾综合征出血热病毒核酸M、S片段cDNA探针。DNA-RNA斑点杂交结果表明，该探针具有肾综合征出血热病毒的特异性，可检出10pgHFRSV-RNA。实验结果表明：抗原阳性鼠体螨10只组、5只组，抗原阴性鼠体螨10只组，游离螨50只组、10只组，抗原阳性鼠肺50mg、100mg组，所抽提RNA与探针杂交结果呈阳性，而抗原阴性鼠体螨5只组抽提RNA的探针检测结果呈阴性。Dig-DNA probes of hemorrhagic fever with renal syndrome virus M S gene fragment were prepared by PCR and random primer labeling.The probes had speciality to HFRSV-RNA and could detect 10pg RNA by RNA-DNA dot-blot hybridization.The results of hybridization showed that positive reactions were found in 5, 10 chigger mites groups from antigenic positive rats,10 mites group from antigenic negative rats,10, 50 free chigger mites, and lung of antigenic positive mice 100mg,500mg.But negative reaction in 5 mites group from antigenic negative mice.

关 键 词：斑点杂交 肾综合征出血热 HFRSV 地高辛素标记 

分 类 号：R512.8[医药卫生—内科学] R373.32[医药卫生—临床医学]