不同组织来源的细胞建立诱导多能干细胞系的研究  

Generation of Induced Pluripotent Stem Cells from Different Tissue Cells

作　　者：范勇[1] 骆玉梅[1] 陈欣洁[1] 黎青[1] 王晓蔓[1] 孙筱放[1] 

机构地区：[1]广东省产科重大疾病重点实验室,广州医学院第三附属医院,广东广州510150

出　　处：《现代生物医学进展》2012年第29期5621-5625,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金(81100473);NSFC-广东联合基金(U1132005);广东省科技厅重大项目(2009A030200010)

摘　　要：目的:比较不同组织来源的细胞生成iPS细胞的效率,获得高效制备iPS细胞的组织类型。方法:通过四种逆转录病毒(OCT4/SOX2/KLF4/c-MYC)转染羊水细胞、绒毛细胞和皮肤成纤维细胞,建立不同组织来源的iPS细胞系。结果:我们建立了羊水、绒毛细胞和皮肤细胞三个不同组织来源的iPS细胞系,并对其多能性基因Oct4、Nanog以及分子表面标记Tra-1-60以及体外分化为三个胚层能力进行鉴定,发现利用羊水细胞建立iPS细胞的效率显著高于绒毛细胞和皮肤细胞。结论:羊水细胞可能是制备iPS细胞的理想细胞类型。ABSTRACT Objective： To investigate the efficiency of different type of cells to generate iPS cells. Methods： Human amniotic fluid cells, chorionic villus ceils and fibroblast cells were infected with retrovirus via ectopic expression of four human factors： OCT4/SOX2/KLF4/c-MYC. The iPS cells were identified by irnmunostaining. Results： Human iPS cells were produced from amniotic fluid cells, chorionie villus cells and fibroblast cells. The iPSCs expressed pluripotency markers such as Oct4, Nanog and Tra-l-60, and they can be differentiated into various somatic cell types in vitro. It was found that generation ofiPS cells from human aumiotic fluid cells were more rapid and efficient than chorionic villus cells and fibroblast cells. Conclusions： Amniotic fluid cells may be a preferred tissue for generating iPSCs. Key Words： Induced pluripotent stem cells; Amniotic fluid cells; Reprogramming efficiency

关 键 词：-诱导多能干细胞 羊水细胞 重编程效率 

分 类 号：Q813[生物学—生物工程] Q75