慢性重度乙型肝炎患者肝组织中NF-κ Bp65和Caspase-3的表达及其意义  

The Expression of NFκ Bp65 and Caspase-3 in Liver Tissues of Chronic Severe Hepatitis B and the Significance

作　　者：郭晓东[1] 张苧月 胡瑾华[1] 熊璐[1] 周光德[1] 赵雨来[1] 

机构地区：[1]解放军第302医院 [2]总后丰台综合仓库(62370部队)药材供应站

出　　处：《现代生物医学进展》2012年第30期5838-5840,5901,共4页Progress in Modern Biomedicine

基　　金：国家"十二五"科技重大专项课题(2012ZX10002-003)

摘　　要：目的:探讨慢性重度乙型肝炎患者肝组织中NF-κBp65和Caspase-3的表达及其意义。方法:选择慢性重度乙型肝炎肝组织标本68例(慢重肝组),正常肝组织标本20例(对照组)。采用免疫组化方法检测肝组织中NF-κBp65和Caspase-3的表达。结果:慢重肝组患者肝组织中NF-κBp65和Caspase-3阳性表达程度均明显强于对照组(P<0.05);慢重肝患者死亡组肝组织中NF-κBp65和Caspase-3阳性表达程度均明显强于存活组(P<0.05)。Spearman相关分析发现,慢重肝组患者肝组织中NF-κBp65和Caspase-3表达之间有明显的正相关性(r=0.449,P<0.05)。结论:NF-κBp65和Caspase-3可能参与了慢性重度乙型肝炎的肝细胞凋亡过程,且具有一定相关性,并对判断患者预后有重要临床价值。Objective： To investigate the expression of NF-κBp65 and Caspase-3 in liver tissues of chronic severe hepatitis B and their significance. Methods： The specimens of the liver tissues in 68 cases with viral hepatitis gravis and 20 cases without liver diseases were detected for the expression of NF-K Bp65 and Caspase-3 by immtmohistochemical staining. Results： The expression of NF-κBp65 and Caspase-3 in viral hepatitis gravis group was significantly higher than those in control group （P〈0.05）. The expression of NF-κBp65 and Caspase-3 in death group was significantly higher than those in living group （P〈0.05）. There was a positive correlation between the expression of NF-K Bp65 and Caspase-3 （r=0.449, P〈0.05）. Conclusion： NF-κBp65 and Caspase-3 with increased expression may participate in the hepatoeyteapoptosis of chronic severe hepatitis B. Therefore, they may be of great value for prognosis judging.

关 键 词：慢性重度乙型肝炎 NF-ΚBP65 CASPASE-3 免疫组织化学 

分 类 号：R512.62[医药卫生—内科学]