驻极体技术改进的脂质体包封血红蛋白及其对体外血液流变学性能的影响  

Improved Liposome Encapsulated Hemoglobin by Electret Technology and Its Influence on the Blood Rheologic Behavior in Vitro

作　　者：邓百明[1] 史向阳[2] 邓劲光[1] 孙曹民[1] 翁维良[3] 王怡[3] 

机构地区：[1]北京理工大学生物功能材料实验室,北京100081 [2]清华大学现代应用物理系,北京100084 [3]中国中医研究院西苑医院,北京100091

出　　处：《生物医学工程学杂志》2000年第1期29-32,36,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目! (39570201)

摘　　要：为了研究脂质体包封血红蛋白 (L iposom e Encapsulated Hemoglobin,L EH)的不同带电性能对体外血液流变性的影响 ,用驻极体技术制备了四种不同带电性能的 L EH。利用热刺激去极化电流法 (Therm ally Stimulat-ed Depolarization Current,TSDC)对它们的驻极体性能进行了测试 ,发现 4#L EH (主峰温度 Tα=12℃ ,主峰电流Iα=- 5 .6× 10 - 9A)的性能最好 ,而且体外血栓成形实验结果表明 ,添加 4#L EH形成血栓重量最小。可见 ,利用驻极体技术对 L EH的表面电性能进行合理的改进 ,就能改善红细胞及血液的流变性能 。To study the influence of different electret properties of Liposome Encapsulated Hemoglobin(LEH) on the blood rheologic behavior in vitro, four kinds of LEH have been prepared. Their electret properties have been determined by using Thermally Stimulated Depolarization Current(TSDC). The results show that 4 # LEH, whose T α is 12℃ and I α is -5.6×10 -9 A, has the best electret property. furthermore, the results of the thrombus formation in vitro also show that the addition of 4 # LEH induces the lightest thrombus figuration in vitro. It is obvious that the rational improvement of the electret property of LEH can meliorate the surface charge of blood and effectively inhibit the formation of thrombus.

关 键 词：生物驻极体 LEH 血栓形成 脂质体 血液流变学 

分 类 号：R318.08[医药卫生—生物医学工程]