极低频弱磁场对PC-12瘤细胞胞内游离钙离子浓度的影响  

Effects of Extremely Low Frequency Weak Magnetic Fields on the Intracellular Free Calcium Concentration in PC-12 Tumor Cells

作　　者：黄春明[1] 叶晖[1] 徐建华[2] 林家瑞[1] 瞿安连[2] 

机构地区：[1]华中理工大学生物工程系,武汉430074 [2]华中理工大学生物物理与生物化学研究所,武汉430074

出　　处：《生物医学工程学杂志》2000年第1期63-65,94,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目!(39670194)

摘　　要：采用极低频弱磁场对鼠嗜铬细胞瘤 PC- 12株系细胞进行照射 ,利用显微荧光技术动态监测胞内游离钙离子浓度 ([(Ca2 + ]i)的变化 ,实验结果表明 :5 0 Hz,10 0 μT的正弦磁场照射引起 [Ca2 + ]i 明显升高 ;而在同等强度的静磁场和 2 0 0 0 Hz正弦磁场照射下 ,[Ca2 + ]i 基本维持不变。进一步的实验说明 [Ca2 + ]i 的升高部分源于细胞外 Ca2 +的跨膜内流 ,部分源于胞内钙库的释放。证实了一定强度下特定频率的极低频弱磁场能够影响钙离子的跨膜运输和胞内钙库的释放 。In this study the effects of the extremely low frequency (ELF) magnetic fields on intracellular Ca 2+ concentration ([Ca 2+ ] i) of rat chromaffinoma PC 12 cells were examined by using a digital fluorescence image microscopy system. A 50 Hz, 100 μT sinusoidal ELF magnetic field increased [Ca 2+ ] i to a marked level, whereas a static field or a field of higher frequency (2000 Hz) did not cause a [Ca 2+ ] i increase. The magnetic field induced [Ca 2+ ] i rise was partly due to an influx of extracellular Ca 2+ and partly due to the release of intracellular Ca 2+ pool. It seemed likely that the Ca 2+ transport ability of the cell membranes was increased by the 50 Hz magnetic field.

关 键 词：极低频弱磁场 游离钙离子 浓度 嗜铬细胞瘤 

分 类 号：R312[医药卫生—基础医学]