人参皂苷Rb_1对术后疲劳综合征大鼠骨骼肌氧化应激的影响  

Effects of Ginsenoside Rb_1 on the Oxidative Stress in the Skeletal Muscles of Rats with Postoperative Fatigue Syndrome

作　　者：谈善军[1,2] 余震[1] 董千铜[1] 章晓东[1] 陈必成[3] 周锋[1] 郭健[1] 裘益辉[1] 

机构地区：[1]温州医学院附属第一医院胃肠外科,浙江325000 [2]南京大学医院临床学院(南京军区南京总医院)解放军普通外科研究所 [3]温州医学院附属第一医院外科学实验室,浙江325000

出　　处：《中国中西医结合杂志》2012年第11期1535-1538,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81171857);浙江省医学支撑学科建设计划(No.11-ZC24);浙江省医药卫生平台重点资助计划(No.2011ZDA018);浙江省中医药科学研究基金计划(No.2011ZB087);2011年浙江省大学生科技创新活动计划(新苗人才计划)项目(No.2011R413046)

摘　　要：目的观察人参皂苷Rb1对术后疲劳综合征(POFS)大鼠骨骼肌氧化应激的影响,探讨其抗疲劳机制。方法采用70%中段小肠切除法建立POFS大鼠模型。通过抓力测试筛选出96只SD大鼠,按体重随机分为对照组、模型组和人参皂苷Rb1干预组(GRb1组,10mg/kg)。分别在术后第1、3、7、10天检测大鼠最大抓力,并检测骨骼肌内丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性。结果 GRb1组较模型组最大抓力在术后第7、10天明显增强(P<0.05),MDA含量在术后第3、7天明显降低(P<0.05),SOD活性同时明显升高(P<0.05)。3组间CAT、GSH-PX活性各时间点无明显变化(P>0.05)。结论人参皂苷Rb1能减轻骨骼肌的氧化应激性损伤,提高骨骼肌抗氧化酶活性,增强骨骼肌功能。这可能是其抗术后疲劳的重要途径。Objective To observe the effects of ginsenoside Rb1 （GRb1） on the oxidative stress in the skeletal muscles of rats with postoperative fatigue syndrome （POFS） and to study its anti-fatigue mechanisms. MethodsThe POFS model was established using resection of 70% of mid-small intestine. Ninety-six Sprague-Dawley （SD） rats were screened using grasping test. The rats were randomly divided into the control group, the model group, and the GRb1 treated group （at 10 mg/kg） by the body weight. The maximum grip strength of rats was detected on the 1st, 3rd, 7th, and 10th day after operation, respectively. The contents of malondialdehyde （MDA）, the activities of superoxide dismutase （SOD）, catalase （CAT）, and glutathione peroxidase （GSH-PX） were detected. Results Compared with the model group, the maximum grip strength was obviously enhanced on the postoperative day 7 and 10 （P0.05）, the MDA content obviously decreased on the postoperative day 3 and 7 （P0.05）, the SOD activity obviously increased in the GRb1 treated group （P0.05）. There was no obvious change in the activities of CAT and GSH-PX among the three groups at each time point （P0.05）. Conclusion GRb1 could reduce the oxidative stress injury in the skeletal muscles, improve the activities of antioxidant enzymes, and enhance the functions of the skeletal muscles in POFS rats, which may be important reasons for fighting against POFS.

关 键 词：人参皂苷RB1 术后疲劳综合征 抓力 氧化应激 

分 类 号：R285.5[医药卫生—中药学]