增龄与运动对大鼠左心室羟基赖氨酰吡啶啉交联水平的影响  

Effects of aged and exercise on hydroxylysylpyridinoline crosslinking concentrations of left ventricule in rats

作　　者：李侠[1] 胡有东[1] 郭燕[1] 

机构地区：[1]徐州医学院附属淮安医院老年病科,淮安223002

出　　处：《中华老年医学杂志》2012年第11期1019-1021,共3页Chinese Journal of Geriatrics

基　　金：江苏省高校自然科学研究计划项目（KJD310234）;江苏省淮安市科技局科技项目（HAS04020）;江苏省淮安市科技支撑计划项目（HAS2011020）

摘　　要：目的研究增龄与运动对大鼠右心室、左心室间隔和左心室游离壁（LVFw）细胞外基质羟基赖氨酰吡啶啉（HP）交联水平的影响。方法采用高效液相色谱法（HPLC）检测Fischer344青年大鼠（青年安静组和青年运动组各10只）、老年大鼠（老年安静组和运动组各10只）右心室、左心室间隔和左心室游离壁细胞外基质HP的交联水平。结果HP交联水平老年安静组左心室间隔和左心室游离壁分别为（0．43±0．03）molHP／molCol和（0．51±0．03）molHP／molCol，较青年安静组（O．23i-0．01）molHP／molCol和（0．22±0．05）molHP／molCol升高（均P〈0．05）。运动不影响青年大鼠组左心室间隔和左心室游离壁的HP交联水平。老年运动组HP交联水平左心室间隔和左心室游离壁分别为（0．22±0．06）molHP／molCol和（0．21±0．07）molHP／molCol，较老年安静组[（0．43±0．03）molHP／molCol和（0．51±0．03）molHP／molC013降低（均P〈0．05）。结论随着年龄的增长，大鼠左心室间隔和左心室游离壁的HP交联水平升高，运动可以阻止HP交联水平在左心室间隔和左心室游离壁的增龄性改变。Objective To study the effects of aged and exercise training on hydroxylysylpyridinoline （HP） crosslinking concentrations of extracellular matrix in right ventricular （RV） ,left ventricular septum （LVS） and left ventrieular free-wall （LVFW） in Fischer 344 rats. Methods We evaluated HP crosslinking concentrations of RV, LVS and LVFW extracellular matrix in young adult versus old Fischer 344 rats using high-pressure liquid chromatography （HPLC）. Rats were assigned to either a sedentary control group or an exercise training group. Results HP crosslinking concentrations in LVS [（0. 23±0. 01） vs. （0.43±0.03）tool HP/mol Col,P〈0. 053 and LVFW [（0. 22±0. 05） vs. （0. 51±0.03） mol HP/mol Col,P〈0. 05] in old sedentary control rats were elevated compared with adult sedentary rats. In adult exercise training rats, training had no effect on HP crosslinking concentration in any of the three regions of the heart. As compared with old sedentary rats, HP crosslinking concentrations in LVS [（0. 22±0. 06） tool HP/mol Col vs. （0.43± 0.03）mol HP/mol Col,P〈0. 05] and LVFW[（0.21±0.07）mol HP/mol Col vs. （0.51±0.03） tool HP/mol Col,P〈0. 053 were decreased in old exercise training rats. Conclusions HP crosslinking concentration are increased with aging in LVS and LVFW of rats, and exercise can prevente this ageassociated change.

关 键 词：年龄因素 运动试验 细胞外基质 

分 类 号：R542.22[医药卫生—心血管疾病]