广西巴马地区与南宁市80岁及以上健康老年人的血清蛋白质谱比较  

Comparison of serum protein profiles between people with successful aged 80 years and over in Bama versus Nanning city

作　　者：伍业光 黄元姣[2] 张国兵 张春林 

机构地区：[1]南宁市社会福利医院,530003 [2]广西医科大学

出　　处：《中华老年医学杂志》2012年第11期1031-1034,共4页Chinese Journal of Geriatrics

基　　金：广西壮族自治区科委自然基金（桂科自0640200）

摘　　要：目的了解广西巴马地区与城市（南宁市）80岁以上健康老年人的血清蛋白质谱状况，为探索巴马地区长寿的奥秘提供新的线索。方法运用表面增强激光解析电离飞行时间质谱（SELDI—TOF—MS）技术，采用弱阳离子交换（CM10）、金属离子螯合（IMAC30-Cu）、强阴离子交换（Q10）、疏水（H50）4种芯片分别检测巴马地区与城市80及岁以上健康老年人的血清蛋白质谱。结果巴马地区与城市健康老年人血清中共检测到蛋白质（或多肽）峰：CM1071个，IMAC30-Cu46个，Q1081个，H5052个；巴马地区与城市健康老年人的血清蛋白质谱峰比较（P％0．05且各蛋白质峰值的均数〉标准差），CM10芯片共检出8个差异蛋白质谱峰，其中2个表达上调、6个表达下调；IMAC—Cu芯片共检出7个差异蛋白质谱峰，其中1个表达上调，6个表达下调；Q10芯片共检出5个差异蛋白质谱峰，均为表达下调；H50芯片共检出6个差异蛋白质谱峰，其中3个表达上调，3个表达下调。结论巴马地区与城市健康老年人的血清蛋白质谱存在一定的差异，且表达下调者居多。Objective To compare the serum protein profiles between people with successful aged 80 years and over in Bama versus Nanning, to identify the possible causes of the long life expectancy in Bama. Methods We evaluated the serum protein profiles using surface-enhanced laser desorption ionization time-of-flight mass spectrometry （ SELDI-TOF-MS ） with 4 different chromatographic surfaces, including the weak cation exchange （CM10）, immobilized metal affinity capture （IMAC） 30-Cu, strong anion exchange （Q10） and hydrophobic surface （H50） protein chip arrays in people with successful aged 80 years and over living in Bama versus in Nanning city. SELDI- TOF-MS had a molecular mass detection range of 2000-50,000 D, with a first signal-to-noise （S/N） ratio of 5, frequency of peak cluster of over 15%, and second S/N ratio of 2 and peak fluctuation of 0.3% （m/z）. Results The total numbers of serum protein （peptide） peaks for people with successful aging in Bama and Nanning were 71 on CM10, 46 on IMAC30-Cu, 81 on Q10, and 52 on HS0. Among subjects from Bama as compared with Nanning （P〈0. 053 mean ）SD for each protein peak）, there were 8 differently expressed protein peaks on CM10, of which 2 were upregulated and 6 downregulated; 7 on IMAC-Cu, of which 2 were upregulated and 6 downregulated; 5 on Q10, all of which were upregulated; and 6 on H50, of which 3 were upregulated and 3 downregulated. Conclusions There are marked differences in the serum protein profiles between people with successful aging living in Bama versus in Nanning. Most of the proteins are downregulated among people aged over 80 years in Bama.

关 键 词：长寿 血蛋白质类 质谱分析法 

分 类 号：R446.1[医药卫生—诊断学]