西洋参多糖PPQI-1～4的分离和表征  

Isolation and Characterization of Four Polysaccharides from American Ginseng (Panax quiquefolium)

作　　者：马秀俐[1] 赵德超 孙允秀[1] 刘举正[1] 郝春艳[2] 刘树莹 

机构地区：[1]吉林大学化学系,长春130023 [2]中国科学院长春应用化学研究所

出　　处：《中草药》2000年第3期165-167,共3页Chinese Traditional and Herbal Drugs

摘　　要：为研究生物活性西洋参多糖的性质 ,采取热水提取乙醇分级沉淀、葡聚糖凝胶分离等手段从西洋参根中分得 4个纯多糖 (PPQI- 1～ 4)。基质辅助激光解吸电离质谱 (MAL DI- MS)测定分子量 ,乙酰化衍生结合GC分析测定糖组成。甲基化结合 GC- MS分析测定糖苷键连接位点。结果表明 ,这 4个多糖化合物都是杂多糖 ,分别由不同比例的阿拉伯糖、半乳糖、葡萄糖、糖醛酸组成 ,糖醛酸含量为 30 %～ 6 0 % ,分子量范围 2～Four bioactive pure polysaccharides of Panax quiquefolium L.(PPQ I 1～4) were isolated by Sephadex chromatography. The molecular weight of the polysaccharides were determined by matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). The sugar compositions were analyzed by gas chromatography (GC). The position of the linkages of the component sugars were determined by methylation and GC MS. The results showed that the four polysaccharides were all uronic acids containning heteropolysaccharide with the component sugar of arabinose, galactose and glucose in different molar ratios. Their molecular weights were in the range of 2×10 4～7×10 4 Dalton.

关 键 词：西洋参 多糖 MALDI-MS GC-MS 中药 

分 类 号：R284[医药卫生—中药学]