补肾健脾化瘀方对老年小鼠免疫功能及自由基代谢的影响  

Effects of Kidney-Supplementing Spleen-Invigorating Blood Stasis-Removing Recipe on Immune Function and Free Radical Metabolism in Old Mice

作　　者：李志新[1] 王传社[1] 李顺成[1] 

机构地区：[1]北京医科大学中西医结合研究所,北京100083

出　　处：《中国医药学报》2000年第1期29-32,共4页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:为验证“正虚挟瘀”是中医衰老的重要原因/机制的可靠性及合理性,我们观察了补肾健脾化瘀方对老年小鼠免疫功能、自由基代谢的影响。方法:老年小鼠连续四周灌服中药后,检测腹腔巨噬细胞产生的IL-1、脾淋巴细胞产生的IL-2、红细胞清除自由基酶(SOD、CAT、GSH-Px)的活性以及肝脑的LPO含量。结果:老年小鼠IL-1、IL-2的产生以及SOD、CAT、GSH-Px的酶活性均明显低于青年小鼠,而肝脑产生的过氧化脂质(LPO)明显增加。给药后,上述指标均可明显得到改善。结论:老年机体确为“虚”和“瘀”并存,进一步为我们的观点提供了实验依据;同时也说明IL-2的产生能力降低除与T细胞本身有关外,还与巨噬细胞、自由基代谢等因素有关。In order to verify the reliability and reasonability of the theory that 'deficiency of Zhenqi complicated with blood stasis is the main cause/mechanism of aging, the effect of the kidney-supplementing spleen-invigorating blood stasis-removing recipe on immune function, free radical metabolism in old mice was studied. After old mice were medicated with decoction for 4 weeks, interleukin-2 (IL-2) production of splenic lymphocytes, interleukin-1 (IL-1) production of peritoneal macrophages, SOD, CAT and GSH-Px activities in ery-throcites. LPO contents in liver & brain were detected. The activities of IL-1, Il-2, SOD, CAT and GSH-Px in old mice were significantly lower than those in the young mice, and LPO contents in liver and brain were higher. After administration, the above-mentioned indexes were obviously improved. The old organism really exists the constitution of 'deficiency' and 'stasis', and further provides and experimental basis for our viewpoint . Meanwhile the decrease of IL-2 production was closely related with the function of macrophage and free radical metabolism, besides lower function of T lymphocyte.

关 键 词：老年小鼠 补肾健脾化瘀法 免疫功能 自由基 

分 类 号：R285[医药卫生—中药学]