人巨细胞病毒pp65对系统性红斑狼疮诱导作用的研究  

The induction of systemic lupus erythematosus by human cytomegalovirus pp65

作　　者：张园[1] 潘阳[2,3] 张括[2] 张瑞[2] 谢洁红[2] 李金明[2] 

机构地区：[1]北京大学第三医院检验科,100191 [2]卫生部北京医院卫生部临床检验中心,100730 [3]北京协和医学院研究生院

出　　处：《中华微生物学和免疫学杂志》2012年第8期689-695,共7页Chinese Journal of Microbiology and Immunology

摘　　要：目的探究人巨细胞病毒（HCMV）pp65是否可以诱发正常C57BL／6小鼠产生系统性红斑狼疮（SLE）相关实验室诊断指标的变化。方法构建HCMVpp65原核表达质粒与真核表达质粒，然后进行HCMVpp65原核蛋白表达与纯化。间接法ELISA检测anti．pp65IgG、dsDNA、抗核抗体（ANA），竞争法ELISA检测血浆中IL-1b、IL-6、TNF-仪浓度。结果成功获得HCMVpp65原核表达蛋白和真核表达质粒，免疫小鼠后血清中anti—pp65、抗dsDNA抗体、ANA、IL-6均有明显上升趋势。结论HCMVpp65可以使C57BL／6小鼠SLE相关检测指标发生明显改变，这一结果有助于进一步研究自身免疫病的发病机制与影响因素。Objective To investigate the potential role of human cytomegalovirus lower matrix phosphoprotein 65 （HCMV pp65） in routine systemic lupus erythematosus （SLE）. Methods The prokary- otic vector pET-28b and eukaryotic vector pcDNA 3.0 were constructed to express the HCMV pp65 protein. All the C57BL/6 mice were inoculated with pp65 eukaryotic vector intramuscularly five times at 2-week in- tervals and then were bled via the retro-orbital vein. Subsequently, indirect ELISA was used to evaluate the concentration of anti-pp65 IgG, anti-dsDNA and ANA. At the same time, IL-lb, IL-6, and TNF-ct were de- termined by competitive ELISA. Results The early onset of autoantibodies and an overexpression of IL-6 were observed in immunized male C57BL/6 mice. Conclusion HCMV pp65 triggers the deregulation of hu- moral immunity in C57BL/6 mice, which indicates that the immune responses induced by HCMV pp65 may be involved in the development of SLE.

关 键 词：自身免疫病 人巨细胞病毒 系统性红斑狼疮 低基质磷蛋白pp65 

分 类 号：R593[医药卫生—内科学]