东方栓孔菌菌丝体生物吸附剂对染料的脱色作用及其吸附机制研究  

Dye decolorization by adsorbents obtained from Trametes orientalis mycelia and investigation of the adsorptive mechanism

作　　者：司静[1] 崔宝凯[1] 李牧洁[2] 

机构地区：[1]北京林业大学微生物研究所,北京100083 [2]北京林业大学水土保持学院,北京100083

出　　处：《菌物学报》2012年第6期890-899,共10页Mycosystema

基　　金：教育部新世纪优秀人才支持计划(No.NCET-11-0585)

摘　　要：利用东方栓孔菌Trametes orientalis菌丝体制得的生物吸附剂,对刚果红、结晶紫、铬天青、亚甲基蓝和中性红5种染料进行了脱色试验,对脱色效果较好的染料进行了影响因子优化,并通过红外光谱分析、化学修饰等方法研究其吸附作用的机制。结果显示:东方栓孔菌菌丝体生物吸附剂对结晶紫有相对较好的脱色效果;在优化的影响因子中,0%–3.0%(m/v)浓度范围内,盐度对染料脱色有促进作用;表面活性剂促使脱色率增加,吐温60浓度为1.5%(v/v)时脱色率可达83.84%;温度为43℃、初始pH为3.0、振荡培养10d后吸附率最高达91.54%。红外光谱分析及化学修饰结果表明菌丝体对染料的吸附作用主要是由它们之间的静电作用力所致。The decolorization of five different dyes （chromazurine, Congo red, crystal violet, methylene blue and neutral red） by the adsorbents obtained from Trametes orientalis mycelia in submerged fermentation was investigated. The optimal conditions and adsorption mechanism were investigated by single-factor experiments, fourier transform infrared spectroscopy （FTIR） analysis and chemical modifications. The results demonstrated that the bioadsorbents had preferable decolorization capacity on crystal violet. In a range of 0%--3.0% （m/V）, salinity had increased effect on dye decolorization. The decolorization capacity was enhanced and up to 83.84% by surfactant Tween 60 with a concentration at 1.5% （V/V）. After 10d, the adsorption rate was up to 91.54% at 43℃ and initial pH3.0 in shaking cultivation. The FTIR analysis and chemical modifications suggested that the biosorption was caused by electrostatic attraction between dye molecules and adsorbents.

关 键 词：白腐真菌 染料吸附 影响因子 生物吸附机制 

分 类 号：X703[环境科学与工程—环境工程]