浓硫酸处理对海滨木槿10个家系种子萌发的影响  

Effects of treating with concentrated sulfuric acid on the seed germination of ten Hibiscus hamabo provenance families

作　　者：王小雪[1,2] 孙海菁[1] 刘芸[2] 陈益泰[1] 冯大兰[3] 李沙[4] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]西南大学资源环境学院,重庆400716 [3]重庆市林业科学研究院三峡库区森林生态保护与恢复重庆市市级重点实验室,重庆400036 [4]中国民主同盟广西壮族自治区委员会,南宁530023

出　　处：《应用生态学报》2012年第11期2968-2974,共7页Chinese Journal of Applied Ecology

基　　金："十一五"国家科技支撑计划项目(2009BADB2B0102);三峡库区森林生态保护与恢复重庆市市级重点实验室项目(CSTC;2007CA1001)资助

摘　　要：以海滨木槿10个家系种子为材料,采用浓硫酸不同时间(0、10、15和20min)处理,测定种子发芽率、发芽势、发芽指数及萌发过程中相对吸水率、可溶性糖、淀粉和α-淀粉酶含量,研究浓硫酸处理对海滨木槿种子萌发的影响以及不同家系间种子萌发差异.结果表明:浓硫酸处理显著提高了海滨木槿种子的发芽率、发芽指数和发芽势,缩短了发芽时间.浓硫酸处理15min后,种子发芽率、发芽势和发芽指数分别为95.7%、91.3%和13.28,萌发时间最短,效果最佳.海滨木槿不同家系间种子发芽率、发芽势和发芽指数差异显著,最高发芽指数(15.13)是最低发芽指数(7.15)的2.12倍,最高发芽势(98.0%)是最低发芽势(55.5%)的1.77倍.浓硫酸处理可加快海滨木槿种子萌发代谢生理生化进程.相对吸水率、可溶性糖和α-淀粉酶均呈单峰曲线变化(先升后降),在萌发初期达到最大值;淀粉含量与种子发芽指数呈显著负相关.The seeds from ten Hibiscus hamabo provenance families were treated with concentrated sulfuric acid for different durations （0, 10, 15, and 20 min）, and the seed germination rate, ger- mination energy, and germination index, as well as the seed relative water adsorption rate, soluble sugar and starch contents, and α-amylase activity during the germination, were determined, aimed to study the effects of treating with concentrated sulfuric acid on the seed germination of H. hamabo and the differences of the seed germination among different H. hamabo provenance families. After treated with concentrated sulfuric acid, the seed germination rate, germination energy, and germi- nation index increased significantly, and the germination time shortened remarkably. Treating with concentrated sulfuric acid for 15 minutes had the best effect, i. e. , the germination rate, germina- tion energy, and germination index were up to 95.7%, 91.3%, and 13.28, respectively, and the germination time was the shortest. The seed germination rate, germination energy, and germination index differed significantly with different provenance families, the highest germination index （15.13） being 2.12 times of the lowest germination index （7.15）, and the highest germinationenergy （98.0%） being 1.77 times of the lowest one （55.5%）. Treating with concentrated sulfuric acid accelerated the physiological and biochemical processes of seed germination, and the relative water absorption rate, soluble sugar content, and ct-amylase activity were decreased after an initial increase, with the maximum at the early stage of germination. There was a significant negative rela- tionship between the seed starch content and the seed germination index.

关 键 词：海滨木槿 自然更新 种子萌发 可溶性糖 淀粉α-淀粉酶 

分 类 号：S792.99[农业科学—林木遗传育种]