高温和湿度对转Bt基因棉叶片Bt蛋白表达的影响  

Effects of high temperature and humidity on leaf Bt protein expression of transgenic Bt cotton

作　　者：张祥[1] 王桂霞[1] 顾超[1] 韩勇[1] 许映飞[1] 陈源[1] 陈德华[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室/农业部长江中下游作物生理生态与栽培重点开放实验室,江苏扬州225009

出　　处：《应用生态学报》2012年第11期3016-3020,共5页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(30971727;31171479);国家农业现代产业技术体系岗位专家项目(CARS-18-20);江苏省高校优势学科建设工程项目;江苏省重点实验室重大项目(10KJA210057);高等学校博士学科点专项科研基金项目(20113250110001)资助

摘　　要：以来源于美国的转Bt基因棉DP410B(常规种)和岱杂1号(杂交种)以及来源于中国的转Bt基因棉泗抗1号(常规种)和泗抗3号(杂交种)为材料,研究高温(37℃)条件下,大气湿度(50%、70%、90%)变化对Bt蛋白表达的影响.结果表明:高温条件下,盛蕾期,温湿度对4个供试品种Bt蛋白表达均无显著影响;盛花期,与对照(温度25～30℃,湿度60%～70%)相比,常规种在50%湿度时叶片Bt蛋白含量显著降低2.6%～3.0%;盛铃期,DP410B、泗抗1号和泗抗3号的Bt蛋白含量在50%湿度处理下比对照显著降低3.3%～5.8%.4个转Bt基因棉品种中,DP410B和岱杂1号的Bt蛋白含量最高,而泗抗1号最低.Different origins Bt cotton cultivars, including DP410B （ conventional cultivar） and Dai- za No. 1 （hybridized cuhivar） from US and Sikang No. 1 （ conventional cultivar） and Sikang No. 3 （ hybridized cultivar） from China, were taken as the test materials to investigate the effects of high temperature （37 ℃ ） and different humidity （50%, 70%, and 90% ） on the leaf Bt protein ex- pression of Bt cotton. At high temperature, temperature and humidity had no significant effects on the leaf Bt protein expression of the euhivars at peak squaring stage. At peak flowering stage, as compared with the control （25-30 ℃ and 60% -70% humidity）, 37 ℃ and 50% humidity de- creased the leaf Bt protein content of conventional cuhivars significantly by 2.6% -3.0%. At peak bolling stage, compared with the control, 37 ℃ and 50% humidity decreased the leaf Bt protein content of DP410B, Sikang No. 1, and Sikang No. 3 significantly by 3.3% -5.8%. Among the four cultivars, DP410B and Daiza No. 1 had the highest leaf Bt protein content, while Sikang No. 1 had the lowest one.

关 键 词：BT棉 BT蛋白 高温 湿度 

分 类 号：S562[农业科学—作物学]