多奈哌齐对血管性痴呆小鼠海马caspase-3表达的影响  

Effect of donepezil on caspase-3 expression in hippocampus of mice with vascular dementia

作　　者：杜江[1] 张德军[2] 王勇[1] 王运杰[1] 

机构地区：[1]中国医科大学附属第一医院神经外科,沈阳110001 [2]庄河市中心医院神经外科,庄河116400

出　　处：《中国组织化学与细胞化学杂志》2012年第5期443-446,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金青年基金(81000565/H0914)

摘　　要：目的探讨盐酸多奈哌齐对血管性痴呆(VD)小鼠海马半胱氨酸蛋白酶-3(cysteinyl aspirate specific-proteinase3,caspase-3)表达的调节作用。方法采用双侧颈总动脉反复缺血-再灌注法制备小鼠VD模型。90只雄性昆明小鼠随机分为3组,假手术组(30只)、模型组(30只)及盐酸多奈哌齐治疗组(30只)。在术后第29天、第30天,经跳台试验和水迷宫试验进行行为学测试,用免疫组化和western blot方法检测各组小鼠海马caspase-3的表达变化。结果盐酸多奈哌齐治疗组小鼠的学习和记忆成绩明显高于VD模型组小鼠(P<0.05);免疫组化和Western blot结果发现,盐酸多奈哌齐治疗组小鼠海马CAl区caspase-3蛋白阳性表达的平均光密度值显著低于VD模型组(P<0.05)。结论盐酸多奈哌齐能够降低VD小鼠海马caspase-3的表达。Objective To study the effect of donepezil on the expression change of cysteinyl aspirate specific-proteinase 3（caspase-3） in the hippocampus of mice with vascular dementia （VD）. Methods The models of VD mice were established through three times of ischemia-reperfusion in bilateral common ca- rotid arteries. 90 Kunming mice were randomly divided into sham operation（30） ,model （30）and treatment groups （treated with donepezil）. The behavioral scores were investigated by step-down and water maze tests at days 29 and 30 respectively. The expression of caspase-3 in the hippocampus of mice was examined by immunohistochemistry and western blot. Results The grades of learning and memory of mice treated with donepezil was obviously increased than those of VD mice（P〈0.05）. The expression of caspase-3 in the hippocampus of mice treated with donepezil was lower than in VD mice（P〈0.05）. Conclusion Donepe- zil can down-regulate the expression of caspase-3 in the hippocampus of mice with vascular dementia.

关 键 词：血管性痴呆 多奈哌齐 CASPASE-3 小鼠 免疫组化 蛋白免疫印记 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]