长期传代后HL-60细胞内5'-核苷酸酶-Ⅱ基因表达与阿糖胞苷耐药性的关系  

Cytosolic 5′-nucleotidase Ⅱ gene expression and Ara-C drug resistance in long-term subcultured HL-60 cells

作　　者：唐晋清[1] 谢晓恬[1] 李威[1] 乔晓红[1] 石苇[1] 周晓迅[1] 

机构地区：[1]同济大学附属同济医院儿科,上海200065

出　　处：《同济大学学报（医学版）》2012年第5期1-4,共4页Journal of Tongji University(Medical Science)

基　　金：上海市科委自然基金(09411963700)

摘　　要：目的观察白血病细胞株(HL-60)及其耐药细胞株(HL-60/R)胞浆5'-核苷酸酶-Ⅱ(CN-Ⅱ)基因表达,以及长期传代后的变化规律,探索CN-Ⅱ与Ara-C疗效及耐药机制的相关性。方法采用RT-PCR方法测定HL-60和HL-60/R的细胞内CN-ⅡmRNA表达,并通过体外培养传代,检测长期传代(5、10、20、30、40、50代)后CN-ⅡmRNA表达变化。结果培养初期结果显示,HL-60和HL-60/R的CN-ⅡmRNA表达水平分别为0.23±0.04和0.47±0.05,HL-60/R的CN-ⅡmRNA表达水平明显高于HL-60(P<0.05)。经长期培养传代至10、20、30、40和50代,各传代检测节点的HL-60和HL-60/R细胞内CN-ⅡmRNA表达水平均无明显变化(P均>0.05)。上述各传代检测节点,HL-60/R的CN-ⅡmRNA表达水平均明显高于HL-60(P均<0.05)。结论 HL-60、HL-60/R细胞中CN-ⅡmRNA表达差异有统计学意义。经长期传代后,上述差异持续存在,提示CN-Ⅱ表达与Ara-C的疗效相关,但在长期传代后,变化并不明显,可能与Ara-C耐药现象的发生无关。Objective To investigate the association of Cytosolic 5＇-nucleotidase 11 （ CN- Ⅱ ） gene expressions with drug resistant in long-term subcultured human leukemia HL-60 cells. Methods Real- Time PCR was used to determinate the CN-Ⅱ mRNA expressions in initial culture of HL-60 snd drug resisitant HL-60R cells and in their different passages （5, 10, 20, 30, 40 and 50）. Results In the initial culture, the CN- Ⅱ mRNA expression levels in HL-60 and HL-60/R cells were 0.23 ± 0.04 and 0.47 ± 0.05 （P 〈 0.05 ）. In the long-term subcultured passages （ 10, 20, 30, 40, 50）, the CN- Ⅱ mRNA expression levels exhibited no change in both HL-60 and HL-60/R cells （ P 〉 0.05 ）. The CN-Ⅱ mRNA expression levels in HL-60/R cell passages （10, 20, 30, 40, 50） were significantly higher than those in HL-60 cell passages （P 〈 0.05 ）. Conclusion CN- Ⅱ mRNA expression is higher in HL-60/R cells than that in HL-60 cells, and the difference continuously exists after long-term cultured, which suggests that the CN-Ⅱ expression is closely related to the development of drug resistance after long term use of Ara-C.

关 键 词：阿糖胞苷 人早幼粒白血病细胞系 胞浆5’-核苷酸酶-Ⅱ 基因表达 

分 类 号：R72[医药卫生—儿科]