活性维生素D_3对输尿管结扎小鼠肾组织胶原Ⅳ蛋白表达的影响  

1,25-dihydroxyvitamin D_3 ameliorates renal interstitial fibrosis in mice

作　　者：李瑾[1] 李微[1] 殷少军[1] 陈维杰[1] 

机构地区：[1]同济大学附属第十人民医院老年科,上海200072

出　　处：《同济大学学报（医学版）》2012年第5期41-44,共4页Journal of Tongji University(Medical Science)

摘　　要：目的通过观察活性维生素D3[1,25-(OH)2VitD3,活性VitD3]对单侧输尿管结扎(unilateral ureteralobstruction,UUO)的CD-1小鼠肾组织胶原Ⅳ蛋白(collagenⅣ)表达的研究,探讨活性VitD3阻止肾组织纤维化作用的机制。方法 42只CD-1小鼠随机分成假手术组(n=6)、UUO组(n=18)、UUO加活性VitD3治疗组(n=18),治疗组再分为0.5、0.25、1.0μg/kg 3个剂量组。UUO组分别于术后(建模后)第3、7、14天处死,其余各组均在术后第7天处死。Western印迹法检测肾脏中胶原Ⅳ蛋白表达量。结果术后第3、7、14天,UUO组肾组织胶原Ⅳ蛋白表达水平都显著低于假手术组,尤其是术后第7天,几乎检测不到胶原Ⅳ蛋白表达。UUO加活性VitD3治疗组的胶原Ⅳ蛋白表达水平显著高于UUO组(P<0.05)。结论活性VitD3对UUO所致CD-1小鼠的肾间质纤维化模型胶原Ⅳ蛋白的表达有一定的促进作用,提示活性VitD3可能具有帮助维持肾小管基底膜完整性的作用。Objective To investigate the effect of 1,25-（OH） 2VitD3 on renal fibrosis in unilateral ureterat obstruction （UUO） of mice. Methods Forty-two male CD-1 mice were randomly assigned to three groups ： Sham-operated group （ n = 6）, UUO group （ n = 18） and UUO plus 1,25-（OH） 2VitD3 _ （0.5, 0.25, 1.0 μg/kg） group （n = 18）. Mice of UUO group were sacrificed 3, 7, 14 d after operation, other groups were sacrificed 7d after operation. The expression of collagen IV in renal tissues was tested by Western blotting. Results Western blotting demonstrated a significant reduction of collagen IV production in UUO group, especially at d7 following UUO; the contents of collagen IV in renal tissue of 1, 25-（OH）2VitD3 group were increased compared to UUO group. Conclusion 1, 25-（OH） 2VitD3 ameliorates renal interstitial fibrosis in the obstructed kidneys, which is associated with the restoration of collagen Ⅳ production.

关 键 词：活性维生素D 3 胶原Ⅳ 肾间质纤维化 

分 类 号：R692[医药卫生—泌尿科学]