半滑舌鳎微卫星标记遗传连锁图谱的构建  

Construction of a microsatellite-based genetic linkage map for Cynoglossus semilaevis

作　　者：赵永伟[1,2] 宋文涛[2] 廖小林[2] 牛余泽[2] 庞仁谊[2] 高峰涛[2] 沙珍霞[2] 陈松林[2] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]中国水产科学研究院黄海水产研究所,农业部海洋渔业可持续发展重点开放实验室,山东青岛266071

出　　处：《中国水产科学》2012年第6期939-945,共7页Journal of Fishery Sciences of China

基　　金：农业部公益性行业科研专项(200903046);山东省泰山学者工程专项

摘　　要：利用全基因组测序方法筛选出微卫星标记,以渤海近海野生个体和人工养殖的半滑舌鳎(Cynoglossus semi-laevis)为亲本交配产生的F1全同胞家系为作图群体,构建了半滑舌鳎雌、雄微卫星标记遗传连锁图谱。用320对引物对父母本和92个F1个体进行遗传分析,共得到288个分离标记,其中包含112个偏分离标记(P<0.05)。其中雌性框架图包含242个标记,分布在21个连锁群上,总长度1 311.9 cM,标记间平均距离为4.9 cM,图谱覆盖率为83.3%;雄性框架图定位标记218个,21个连锁群,总长度1 316.2 cM,标记间平均距离为5.5 cM,覆盖率为82%。半滑舌鳎遗传连锁图谱的构建为半滑舌鳎重要经济性状QTL定位、分子标记辅助育种和性别控制奠定了重要基础。Female and male linkage maps were constructed using SSR markers based on a full sub-family of the tongue sole（Cynoglossus semilaevis）.A total of 320 markers were generated in two parents and 92 F1 progenies of the mapping family by using 320 screened SSR primer combinations containing 112 segregation distortion mark-ers（P0.05）.The female linkage map included 21 linkage groups and 242 pairs of SSR markers spanning 1311.9 cM with the average marker interval of 4.9 cM and the observed coverage was 83.3%.The male linkage map in-cluded 21 linkage groups and pairs of 218 SSR markers spanning 1 316.2 cM with the average marker interval of 5.5 cM and the observed coverage was 82.0%.The genetic linkage maps are very significant for QTL analyses marker-assisted selection（MAS） breeding programs for economically important traits and sex control in C.semilaevis.

关 键 词：半滑舌鳎 微卫星 遗传连锁图谱 分子标记辅助育种 

分 类 号：Q959[生物学—动物学]