利用噬菌体随机肽库筛选抗原模拟表位的研究进展  

A Review on Screening Mimicking Epitope of Antigen from Phage Random Peptide Library

作　　者：裴亚峰[1,2] 胡骁飞[1] 王耀[2] 侯玉泽[2] 张改平[1] 邓瑞广[1] 

机构地区：[1]河南省农业科学院农业部动物免疫学重点实验室/河南省动物免疫学重点实验室,河南郑州450002 [2]河南科技大学食品与生物工程学院,河南洛阳471003

出　　处：《河南农业科学》2012年第10期14-18,共5页Journal of Henan Agricultural Sciences

基　　金：农业部公益性行业(农业)科研专项(201203040);河南省农业科学院科技发展及示范推广专项

摘　　要：噬菌体随机肽库技术是将编码外源多肽的DNA序列插入到噬菌体外壳蛋白结构基因的适当位置,使外源基因随外壳蛋白结构基因的表达而表达,被展示的外源多肽可保持相对独立的空间结构和生物活性,是一种基因型与表达型的统一。近年来,噬菌体随机肽库技术被广泛应用于各种抗原的模拟表位筛选中,并取得了显著成果。综述了利用噬菌体随机肽库技术在抗原模拟表位筛选中的研究进展。In this paper, the screening mimicking epitope of antigen from phage random peptide library was reviewed. The phage random peptide library is a technology by inserting the DNA sequences of polypeptide into the phage coat protein gene at the proper position and the heterolo- gous gene is expressed accompanied with the coat protein. Then,the displayed heterologous poly- peptide can be kept relatively independent spatial structure and biological activity,which is a unity of genotype and phynotype. In recent years, phage random peptide library has been widely used for screening the mimicking epitope of various antigen and has made remarkable achievements.

关 键 词：噬菌体随机肽库 噬菌体展示技术 模拟表位 抗原 

分 类 号：Q786[生物学—分子生物学]