水稻根部蛋白质组样品制备的方法研究  

A Study on Sample Preparation Method of Rice Root Proteome

作　　者：袁丽环[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《河南农业科学》2012年第10期25-27,共3页Journal of Henan Agricultural Sciences

基　　金：山西省"十一五"教育规划项目(GH-06044)

摘　　要：为了选择适用于水稻根部蛋白质组分析的样品制备方法,以水稻苗期幼嫩根尖为材料,采用TCA/丙酮沉淀法、Tris法、尿素/硫脲法对水稻根部总蛋白质进行提取并比对,通过Bradford法、SDS PAGE电泳技术对蛋白质含量和种类进行分析。结果显示,Tris法提取出的水稻根部蛋白质含量和种类明显少于其他2种方法;尿素/硫脲法和TCA/丙酮沉淀法提取的蛋白质种类和含量都较多,其中,尿素/硫脲法的蛋白质图谱中蛋白条带多,染色深而且很清晰,尤其适用于较低分子量蛋白质的提取,TCA/丙酮沉淀法则适于高分子量蛋白质的提取。因此,进行水稻根部总蛋白质组分析时,可选用尿素/硫脲法。In order to develop the sample preparation methods for rice root proteome analysis,the TCA/acetone precipitation method, direct extraction method and urea/thiourea method were used to extract the rice root protein,and the protein contents and types of samples were analyzed using Bradford method and SDS-PAGE electrophoresis. The results indicated that the direct extraction method was the worst among the three methods, giving the least protein bands and protein con- tent ~ the TCA/acetone precipitation method and the urea/thiourea method were the optimal meth- ods,both producing more protein bands, while the latter showed more protein types and more clear bands, especially for the low-molecular weight proteins, while the TCA/acetone precipitation method was more suitable for the preparation of high molecular weight proteins. It was suggested that different methods had different specialties and the urea/thiourea method was the optimal method for total protein extraction of rice roots.

关 键 词：水稻 根部蛋白质 提取方法 SDS—PAGE 

分 类 号：Q503[生物学—生物化学] S511[农业科学—作物学]