灯盏花素对大鼠肝纤维化分泌型磷脂酶A_2和细胞因子的影响  

Effect of breviscapine on rat liver fibrosis secretory phospholipase A_2 and cell factor

作　　者：马玉倩[1] 李丽[1] 武凡[1] 

机构地区：[1]河北省涿州市医院检验科,072750

出　　处：《临床合理用药杂志》2012年第34期25-26,共2页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的探讨灯盏花素通过抑制分泌性磷脂酶A2(sPLA2)及其相关细胞因子对肝纤维化的影响。方法将健康雄性Wistar大鼠18只随机分为3组:灯盏花素组、CCl4模型组及对照组各6只。PBS灌胃作为对照组;5%CCl4橄榄油按5ml/kg灌胃,每3天1次共15次,作为CCl4模型组;每次CCl4灌胃同时腹腔注射灯盏花素5mg/kg,作为灯盏花素组。于预定时间处死大鼠,对大鼠肝脏分别进行HE染色、电镜观测、sPLA2免疫组化,并且检测大鼠血清sPLA2、肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)、血清Ⅲ型前胶元(PCⅢ)和透明质酸(HA)水平。结果灯盏花素组胶原纤维、丙氨酸氨基转移酶(ALT)、HA及PCⅢ水平高于CCl4模型组,CCl4模型组高于对照组,差异均有统计学意义(P<0.01)。灯盏花素组第2周、第4周、第6周时PGE2、TNF-α及sPLA2水平低于CCl4模型组,CCl4模型组高于对照组,差异均有统计学意义(P<0.01)。灯盏花素组sPLA2阳性细胞数为(10.2±3.5)个/mm2少于CCl4模型组的(37.2±7.3)个/mm2,CCl4模型组多于对照组的(1.3±0.8)个/mm2,差异均有统计学意义(P<0.01)。结论灯盏花素具有抗肝纤维化作用,其作用机制与抑制sPLA2、TNF-α、PGE2水平相关。Objective To explore the effect of breviscapine on liver fibrosis by inhibiting secretory phospholipase A2 （sPLA2 ） and cell factor. Methods 18 healthy male Wistar adult rats were randomly divided into 3 groups： Breviscapine group, CC14 model group and control group, each of 6 rats. The group with PBS was set as control group; The group with 5 % CC14 olive oil gavaged by 5ml/kg, three times a day,total of fifteen times ,was set as CC14 model group;At the same time with CC14 gavaged,intraperitoneal injected breviscapine 5mg/kg, was set as breviscapine group. Rats were killed at the scheduled time. Rat liver were underwent with HE staining, electron microscopy determination and the sPLA2 immune staining. Detected the serum sPLA2 ,tumor necrosis factor-α（TNF-α） ,prostaglandin E2 （PGE2 ） ,serum procollagen type Ill collagen（ PC Ⅲ） and hyaluronic acid（HA） level. Results The collagen fibers, alanine aminotransferase enzyme（ALT）, HA and PC m level of breviscapine group were higher than CC14 model group;CC14 model group was higher than control group,the difference were statis- tically significant（P 〈0. 01）. The PGE2 ,TNF-α and sPLA2 level at the second week,fourth week, sixth week of breviscapine group were lower than those of CC14 model group; CC14 model group was higher than control group, the difference were statistics significant（ P 〈0.01 ）. The sPLA2 positive cells[ （ 10.2±3.5 ）/mm2 ] of breviscapine group was less than CC14 model group [（37.2±7.3）/mm2];More than control group[（1.3±0.8）/mm2] （P〈0.01）. Conclusion Breviseapine can inhibit theprocedure of hepatic fibrosis through the possible mechanism of reduction the level of sPLA2 ,TNF-α ,PGE2.

关 键 词：灯盏花素 肝脏 纤维化 分泌型磷脂酶A2 

分 类 号：R285.5[医药卫生—中药学]