脂溶性CdSe/ZnS量子点脂质体复合物的制备及表征  

Preparation and Characterization of Liposome Coated Hydrophobic CdSe/ZnS Core-shell QDs

作　　者：邵太丽[1,2] 李萍[1] 赵志刚[1] 宋雪飞[2] 朱昌青[2] 

机构地区：[1]皖南医学院药学系,安徽芜湖241002 [2]安徽师范大学化学与材料学院,安徽芜湖241002

出　　处：《发光学报》2012年第11期1187-1191,共5页Chinese Journal of Luminescence

基　　金：省教育厅自然科学基金(KJ2011Z397);校中青年科研基金(WK201020)资助项目

摘　　要：在油相中成功合成了脂溶性CdSe/ZnS核壳量子点纳米粒,粒径平均为4.5 nm,量子产率达29%,发射波长为540 nm。通过薄膜分散法,以蛋黄卵磷脂、胆固醇为膜材,将脂溶性的CdSe/ZnS核壳量子点包覆于脂质体磷脂双分子层中,由于磷脂分子的两亲性,使得脂溶性的CdSe/ZnS核壳量子点同时又具有亲水性。通过透射电镜对脂质体形态进行了表征,倒置荧光显微镜证实了发光CdSe/ZnS核壳量子点成功包埋于脂质体双分子层中,包裹的发光CdSe/ZnS核壳量子点具有更稳定的发光及抗光漂白性质。In the organic phase,hydrophobic CdSe/ZnS core-shell quantum dots（QDs） were synthesized successfully using a free-phosphine and non-injecction method.The transmission electron microscopy（TEM） shows CdSe/ZnS core-shell nanocrstals with an average diameter of 4.5 nm.The fluo-rescence quantum yield of the QDs,calibrated with fluorescein using a 470 nm laser,was found to be 29%,which was greatly increased compared with bare CdSe QDs,indicating strong photoluminescence.The maximum emission wavelength of QDs was 540 nm at excitation of 410 nm.Through the thin-film evaporation method,using the L-α-phosphatidylcholine and Cholesterol,the hydrophobic CdSe/ZnS core-shell QDs were successfully embedded in the two-layer of liposome.As the amphipathy of phospholipid molecules,rendering the luminescent hydrophobic CdSe/ZnS core-shell QDs hydrophilia,CdSe/ZnS core-shell QDs can be transferred in water without extra surface combining reagents.The morphology of liposome was characterized by TEM using negatively stained method,the process of CdSe/ZnS core-shell QDs embedded in the phospholipid two-layer was confirmed by inverted fluorescent microscopy under the UV light.The result indicates that CdSe/ZnS core-shell QDs embedded in the phospholipid two-layer have more photostability and more resistant photobleaching.

关 键 词：CDSE ZnS核壳量子点 脂质体 荧光 

分 类 号：O482.3[理学—固体物理]