沙门菌质粒对细菌生物膜形式的影响  

The effect of Salmonella typhiplasmid on biofilm formation

作　　者：廖莉[1] 吴淑燕[1] 李嫄渊[1] 刘珍[1] 黄瑞[1] 

机构地区：[1]苏州大学医学部病原生物学系,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2012年第5期609-612,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金(30972768);江苏省自然科学基金(BK2011286);教育部高等学校博士学科点专项科研基金(2010320111009)

摘　　要：目的研究伤寒沙门菌质粒pRST98对生物膜形成的影响。方法将携带pRST98的野生型伤寒沙门菌于30℃和37℃培养3 d,用半定量法确定生物膜形成的适宜温度。将稀释的菌液于30℃分别培养12 h、1 d、2 d、3 d、4 d和5 d,用半定量法确定生物膜成熟的时间。分别用携带pRST98的野生型伤寒沙门菌,消除pRST98的突变株及pRST98的回补株建立生物膜模型,用结晶紫染色法,半定量法和扫描电镜观察三种株受试菌形成生物膜的能力。结果在30℃培养时细菌生物膜形成能力高于37℃,此温度为沙门菌生物膜形成的适宜温度;3 d时生物膜趋于成熟;野生株和回补株形成生物膜的能力显著高于突变株。结论伤寒沙门菌质粒pRST98与该菌生物膜形成密切相关。Objective To investigate the effect of Salmonella typhi（S.typhi） plasmid on the formation of biofilm.Methods Wild-type S.typhi strain carrying plasmid pRST98 was cultured at 30℃ or 37℃ for 3 d to determine the optimum temperature of biofilm formation using semi-quantitative method.Then the strain was cultured at 30℃ for 12 h,1 d,2 d,3 d,4 d and 5 d to determine the biofilm mature time using semi-quantitative method.Wild-type S.typhi strain carrying plasmid pRST98,pRST98-deletion S.typhi strain and pRST98-complemented S.typhi strain were used and then the biofilm formation ability of these three strains was compared by using crystal violet staining method,semi-quantitative method and scanning electron micrographs.Results The ability of biofilm formation was better at 30℃,which is the optimum temperature of Salmonella biofilm formation.Biofilm became mature after cultured for 3 d.The ability biofilm formation by wild-type and pRST98-complemented S.typhi strain to form biofilm was stronger than that of pRST98-deletion S.typhi strain.Conclusion Plasmid pRST98 is closely related to biofilm formation of S.typhi.

关 键 词：伤寒沙门菌 质粒 生物膜 

分 类 号：R392.1[医药卫生—免疫学]