共培养体系中TGF-β1沉默大肠癌LoVo细胞对巨噬细胞IL-12和TNF-α分泌的影响  

Effects of TGF-β1 silencing of colon cancer LoVo cell on IL-12 and TNF-α secretion by the co-cultured macrophages

作　　者：顾新华[1] 周辰超[2] 谭纪伏[1] 袁政[1] 柳燕东[2] 罗家乐[2] 季清华[2] 王益[2] 陈城[2] 高蓓[2] 王守立[2] 

机构地区：[1]苏州市立医院普外科,江苏苏州215002 [2]苏州大学医学部病理学系,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2012年第5期619-622,660,共5页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(81272738);苏科技[2011]231号SYSD2011112;苏州大学大学生课外学术科研基金项目(KY2012101A);苏州大学大学生创新实验计划(5731515611)

摘　　要：目的研究TGF-β1沉默人大肠癌LoVo细胞对人巨噬细胞系28SC免疫功能的影响。方法将构建的TGF-β1siRNA稳定表达LoVo细胞与人巨噬细胞系28SC共培养,酶联免疫吸附实验检测培养液中IL-12和TNF-α水平,同时设立对照组空载体pSUPER-neo-gfp稳定转染LoVo细胞+28SC。结果 TGF-β1siRNALoVo+28SC组IL-12和TNF-α分泌量高于对照组(均P<0.05);TGF-β1+28SC组IL-12和TNF-α分泌量与LoVo+28SC组之间无明显差异(P>0.05)。结论大肠癌LoVo细胞分泌的TGF-β1能通过影响IL-12和TNF-α的分泌抑制巨噬细胞的免疫功能,有望通过阻断TGF-β1信号转导改善肿瘤免疫微环境抑制大肠癌的发生发展。Objective To study the effects of TGF-β1 silencing of human colon cancer LoVo cell on immune function of human macrophages cell line 28SC.Methods LoVo cells stably expressing TGF-β1 siRNA were cultivated together with human macrophages cell line 28SC.The levels of IL-12 and TNF-α in culture medium were detected by enzyme linked immunosorbent assay（ELISA）.While a control group was established.Results The concentrations of IL-12 and TNF-α in TGF-β1siRNA LoVo＋28SC group were higher than those in control group（P0.05）.The concentrations of IL-12 and TNF-α in TGF-β1＋28SC group and control group had no significant difference（P0.05）.Conclusion TGF-β1 secreted by colon cancer LoVo cells can inhibit the immune function of macrophages through affecting the secretion of IL-12 and TNF-α.And it is hopeful to inhibit the development of colon cancer through blocking TGF-β1 signal transduction.

关 键 词：转化生长因子Β1 大肠癌 肿瘤微环境 

分 类 号：R614.2[医药卫生—麻醉学] R614.4[医药卫生—外科学]