鲤45S rDNA的染色体荧光原位杂交定位  

Chromosomal localization of 45S rDNA in common carp by fluorescence in situ hybridization

作　　者：赵紫霞[1] 邓海霞[2] 徐鹏[1] 张研[1] 李炯棠[1] 孙效文[1] 

机构地区：[1]中国水产科学研究院生物技术研究中心,北京100141 [2]大连海洋大学水产与生命学院,辽宁大连116023

出　　处：《大连海洋大学学报》2012年第5期457-463,共7页Journal of Dalian Ocean University

基　　金：中国水产科学研究院院部中央级公益性科研院所基本科研业务费专项资金资助项目(2010C019);国家"863"计划项目(2011AA100401)

摘　　要：应用荧光原位杂交技术,通过设计位于5.8S rDNA、18S rDNA和非转录IGS区域的3条探针CAAG1191、CAAG1845和CAAG3602,分别对散鳞镜鲤Cyprinus carpio var.scattered mirror和松浦鲤Cyprinuscarpio Songpu的45S核糖体DNA(ribosomal DNA,rDNA)进行染色体定位及共定位。结果表明:45S rDNA均位于两品种鲤一对近端着丝粒染色体的短臂末端,具有染色体特异性,表明45S rDNA序列的探针能够在鲤细胞遗传学研究中用于标识其所在染色体,并与鲤遗传连锁图谱中长度为227 cM的1号连锁群相对应;两品种鲤的染色体数目均为2n=100,45S rDNA在鲤基因组内仅定位于一对同源染色体,不存在复制位点,证实了鲤基因组在全基因组复制事件之后又经历了重新二倍化过程。Fluorescence in situ hybridization was carried out in chromosomes of scattered mirror carp and Songpu carp（Cyprinus carpio L.） to localize 45S ribosomal DNA（rDNA）.Three hybridization probes CAAG1191,CAAG1845,and CAAG3602 were designed for chromosome localization and co-localization from 5.8S rDNA,18S rDNA,and non-transcribed IGS region.The 45S rDNA was found to be localized in only one pair of homologous chromosomes in both common carp.The 45S rDNA derived probes as chromosome specific markers were able to be utilized for chromosome identification in kinds of cytogenetic research for common carp,indicating that the chromosomes related to the 227 cM linkage group 1 in common carp genetic linkage map.Diploid chromosome number of both carp was 2n=100,and no duplication loci were observed in 45S rDNA.The findings suggested that common carp genome was under a re-diploidization process after its whole genome duplication event.

关 键 词：鲤 45S RDNA 荧光原位杂交 染色体定位 

分 类 号：Q343.1[生物学—遗传学] S917.4[农业科学—水产科学]